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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Sánchez Sánchez, R. | es_ES |
dc.contributor.author | Ferre, Ignacio | es_ES |
dc.contributor.author | Re, M. | es_ES |
dc.contributor.author | Regidor-Cerrillo, Javier | es_ES |
dc.contributor.author | Blanco Murcia, J. | es_ES |
dc.contributor.author | Ferrer, L. M. | es_ES |
dc.contributor.author | Navarro, Teresa | es_ES |
dc.contributor.author | Pizarro, M. | es_ES |
dc.contributor.author | González Huecas, M. | es_ES |
dc.contributor.author | Tabanera, E. | es_ES |
dc.contributor.author | Benavides, Julio | es_ES |
dc.contributor.author | Ortega Mora, Luis M. | es_ES |
dc.date.accessioned | 2018-07-25T10:43:40Z | - |
dc.date.available | 2018-07-25T10:43:40Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Veterinary Research, 49:42 (2018) | es_ES |
dc.identifier.issn | 0928-4249 | - |
dc.identifier.uri | http://hdl.handle.net/10261/167901 | - |
dc.description | 15 páginas, 2 tablas, 7 figuras. | es_ES |
dc.description.abstract | Experimental infections in pregnant sheep have been focused on studying the effect of the time of challenge on the outcome of N. caninum infection, whereas the impact of the dose and route of challenge has not been studied in depth. Therefore, clinical outcome, immune responses, parasite detection and burden, and lesion severity in placental tissues and foetal brains were investigated in 90-day-pregnant sheep inoculated intravenously with 10(5) (G1), 10(4) (G2), 10(3) (G3), or 10(2) (G4) tachyzoites or subcutaneously with 10(4) (G5) tachyzoites of the virulent Nc-Spain7 isolate and an uninfected group (G6). Comparing challenge doses, G1 was the only group that had 100% abortion. Likewise, IFN gamma levels in G1 increased earlier than those in other intravenously infected groups, and IgG levels on day 21 post-infection (pi) were higher in G1 than those in other intravenously infected groups. Concerning vertical transmission, G1 shows a higher parasite burden in the foetal brain than did G2 and G3. Comparing routes of administration, no differences in foetal survival rate or parasite load in the foetal brain were found. Although G2 had higher IFN gamma levels than G5 on day 10 pi, no differences were found in humoral immune responses. Because the outcome after intravenous infection with 10(5) tachyzoites was similar to that observed after intravenous infection with 10(6) tachyzoites used in a previous work (100% abortion and vertical transmission), we conclude that it may be reasonable to use 10(5) tachyzoites administered by the intravenous route in further experiments when assessing drugs or vaccine candidates. | es_ES |
dc.description.sponsorship | We gratefully acknowledge Neus Callizo Esteve from Clinical Veterinary Hospital (Complutense University of Madrid, Spain) and Jose Maria Gonzalez (University of Zaragoza, Spain) for their excellent technical assistance. The Animal Experimentation Service (SEA) at the University of Zaragoza is acknowledged for using their facilities to carry out the reproduction programme. Roberto Sanchez Sanchez is supported by a fellowship from the Spanish Ministry of Education, Culture and Sports (MECD) as a part of the Programme of Training of University Teaching Staff (FPU, Grant Number FPU13/03438). This work was supported by the Ministry of Economy and Competitiveness (AGL2016-75935-C2) and Community of Madrid, Spain (PLATESA, S2013/ABI2906). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-75935-C2 | es_ES |
dc.relation | S2013/ABI2906/PLATESA-CM | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Host-parasite relationship | es_ES |
dc.subject | Bovine neosporosis | es_ES |
dc.subject | Toxoplasma-gondii | es_ES |
dc.subject | Fetal-death | es_ES |
dc.subject | Immune-responses | es_ES |
dc.subject | In-ovine | es_ES |
dc.subject | Abortion | es_ES |
dc.subject | Cattle | es_ES |
dc.subject | Pathogenesis | es_ES |
dc.subject | Gestation | es_ES |
dc.title | Influence of dose and route of administration on the outcome of infection with the virulent Neospora caninum isolate Nc-Spain7 in pregnant sheep at mid-gestation | es_ES |
dc.type | artículo | es_ES |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1186/s13567-018-0539-5 | es_ES |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.contributor.funder | Ministerio de Educación, Cultura y Deporte (España) | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | Comunidad de Madrid | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003176 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.contributor.orcid | Benavides, Julio [0000-0001-9706-100X] | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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Sánchez, R._Influence of dose_Veterinary research_2018.pdf | Artículo principal | 1,46 MB | Adobe PDF | Visualizar/Abrir |
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