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dc.contributor.authorYeste Lozano, Josées_ES
dc.contributor.authorMartínez Gimeno, Lauraes_ES
dc.contributor.authorIlla, Xavies_ES
dc.contributor.authorLaborda, Pabloes_ES
dc.contributor.authorGuimerà, Antones_ES
dc.contributor.authorSánchez Marín, Juan Pes_ES
dc.contributor.authorVilla, Rosaes_ES
dc.contributor.authorGiménez, Ignacioes_ES
dc.identifier.citationBiotechnology and Bioengineering 115(6): 1604-1613 (2018)es_ES
dc.description.abstractTransepithelial electrical measurements in the renal tubule have provided a better understanding of how kidney regulates electrolyte and water homeostasis through the reabsorption of molecules and ions (e.g., H2O and NaCl). While experiments and measurement techniques using native tissue are difficult to prepare and to reproduce, cell cultures conducted largely with the Ussing chamber lack the effect of fluid shear stress which is a key physiological stimulus in the renal tubule. To overcome these limitations, we present a modular perfusion chamber for long-term culture of renal epithelial cells under flow that allows the continuous and simultaneous monitoring of both transepithelial electrical parameters and transepithelial NaCl transport. The latter is obtained from electrical conductivity measurements since Na+ and Cl- are the ions that contribute most to the electrical conductivity of a standard physiological solution. The system was validated with epithelial monolayers of raTAL and NRK-52E cells that were characterized electrophysiologically for 5 days under different flow conditions (i.e., apical perfusion, basal, or both). In addition, apical to basal chemical gradients of NaCl (140/70 and 70/140 mM) were imposed in order to demonstrate the feasibility of this methodology for quantifying and monitoring in real time the transepithelial reabsorption of NaCl, which is a primary function of the renal tubule.es_ES
dc.description.sponsorshipThis work is part of the requirements to achieve the PhD degree in Electrical and Telecommunication Engineering at the Universitat Autònoma de Barcelona, and it was supported by grants from Ministerio de Economía y Competitividad (MINECO) (SAF2014-62114-EXP, DPI2015-65401-C3-3-R, and DPI2011-28262-C04-02). This work has made use of the Spanish ICTS Network MICRONANOFABS partially supported by MINECO and the ICTS 'NANBIOSIS', more specifically by the Micro-Nano Technology Unit of the CIBER in Bioengineering, Biomaterials & Nanomedicne (CIBER-BBN) at the IMB-CNM.es_ES
dc.publisherJohn Wiley & Sons-
dc.subjectTransepithelial electrical resistancees_ES
dc.subjectcell layer capacitancees_ES
dc.subjectmicrofluidic cell culturees_ES
dc.subjecttransepithelial ion fluxeses_ES
dc.subjectsodium reabsorptiones_ES
dc.subjectrenal epitheliumes_ES
dc.titleA perfusion chamber for monitoring transepithelial NaCl transport in an in vitro model of the renal tubulees_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España)es_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidJose Yeste [0000-0001-7540-7305]es_ES
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