Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/167812
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Preliminary crystallographic analysis of the ankyrin-repeat domain of Arabidopsis thaliana AKT1: Identification of the domain boundaries for protein crystallization |
Autor: | Chaves Sanjuán, Antonio CSIC ORCID; Sánchez-Barrena, María José CSIC ORCID; González-Rubio, Juana M. CSIC; Albert, Armando CSIC ORCID | Fecha de publicación: | 2014 | Citación: | Acta Crystallographica Section F:Structural Biology Communications 70: 509- 512 (2014) | Resumen: | The Arabidopsis thaliana K+ transporter 1 (AKT1) participates in the maintenance of an adequate cell potassium (K+) concentration. The CBL-interacting protein kinase 23 (CIPK23) activates AKT1 for K+ uptake under low-K+ conditions. This process is mediated by the interaction between the cytosolic ankyrin-repeat (AR) domain of AKT1 and the kinase domain of CIPK23. However, the precise boundaries of the AR domain and the residues responsible for the interaction are still unknown. Here, the optimization procedure to obtain an AR domain construct suitable for crystallization and the preliminary crystallographic analysis of the obtained crystals are reported. The crystals belonged to space group P21212, with unit-cell parameters a = 34.83, b = 65.89, c = 85.44 Å, and diffracted to 1.98 Å resolution. © 2014 International Union of Crystallography All rights reserved. | URI: | http://hdl.handle.net/10261/167812 | DOI: | 10.1107/S2053230X14005093 | Identificadores: | doi: 10.1107/S2053230X14005093 issn: 2053-230X |
Aparece en las colecciones: | (IQF) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
pg5035.pdf | 458,99 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
3
checked on 24-feb-2024
SCOPUSTM
Citations
3
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
3
checked on 22-feb-2024
Page view(s)
226
checked on 24-abr-2024
Download(s)
147
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.