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dc.contributor.authorReinoso-Colacio, Mercedes-
dc.contributor.authorGarcía-Rodríguez, Fernando-
dc.contributor.authorGarcía-Cañadas, Marta-
dc.contributor.authorAmador-Cubero, Suyapa-
dc.contributor.authorGarcía-Pérez, José Luis-
dc.contributor.authorToro, Nicolás-
dc.date.accessioned2018-07-09T08:47:20Z-
dc.date.available2018-07-09T08:47:20Z-
dc.date.issued2015-
dc.identifierdoi: 10.1038/srep12716-
dc.identifierissn: 2045-2322-
dc.identifier.citationScientific Reports 5 (2015)-
dc.identifier.urihttp://hdl.handle.net/10261/167449-
dc.description.abstractGroup II introns are mobile retroelements that self-splice from precursor RNAs to form ribonucleoparticles (RNP), which can invade new specific genomic DNA sites. This specificity can be reprogrammed, for insertion into any desired DNA site, making these introns useful tools for bacterial genetic engineering. However, previous studies have suggested that these elements may function inefficiently in eukaryotes. We investigated the subcellular distribution, in cultured human cells, of the protein encoded by the group II intron RmInt1 (IEP) and several mutants. We created fusions with yellow fluorescent protein (YFP) and with a FLAG epitope. We found that the IEP was localized in the nucleus and nucleolus of the cells. Remarkably, it also accumulated at the periphery of the nuclear matrix. We were also able to identify spliced lariat intron RNA, which co-immunoprecipitated with the IEP, suggesting that functional RmInt1 RNPs can be assembled in cultured human cells.-
dc.description.sponsorshipThis work was supported by research grants CSD 2009–0006 from the Consolider-Ingenio, BIO2011-24401 and BIO2014-51953-P from the Spanish Ministerio de Economía y Competitividad all including ERDF (European Regional Development Funds). We thank Dr. Antonio Barrientos Durán for technical advice. MRC was supported by an FPI Ph.D grant. J.L.G.P´s laboratory is supported by CICE-FEDER-P09-CTS-4980, CICE-FEDER-P12-CTS-2256, Plan Nacional de I+D+I 2008–2011 and 2013–2016 (FIS-FEDER-PI11/01489 and FIS-FEDER-PI14/02152), PCIN-2014-115-ERA-NET NEURON II, the European Research Council (ERC-Consolidator ERC-STG-2012-233764) and by an International Early Career Scientist grant from the Howard Hughes Medical Institute (IECS-55007420).-
dc.publisherNature Publishing Group-
dc.relationMINECO/BIO2011-24401; MINECO/BIO2014-51953-P-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.titleLocalization of a bacterial group II intron-encoded protein in human cells-
dc.typeartículo-
dc.identifier.doihttp://dx.doi.org/10.1038/srep12716-
dc.date.updated2018-07-09T08:47:20Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderEuropean Commission-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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