English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/167382
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Regulation by S-nitrosylation of the Calvin-Benson cycle fructose-1,6-bisphosphatase in Pisum sativum

AutorSerrato, Antonio Jesús; Romero-Puertas, María C.; Lázaro-Payo, Alfonso; Sahrawy, Mariam
Palabras claveFructose-1,6-bisphosphatase
GSNO
Redox regulation
S-nitrosylation
Calvin-Benson cycle
Pisum sativum
Fecha de publicación2018
EditorElsevier
CitaciónRedox Biology 14: 409- 416 (2018)
ResumenRedox regulation is of great importance in chloroplasts. Many chloroplast enzymes, such as those belonging to the Calvin-Benson cycle (CBC), have conserved regulatory cysteines which form inhibitory disulphide bridges when physiological conditions become unfavourable. Amongst these enzymes, cFBP1, the CBC fructose-1,6-bisphosphatase (FBPase) isoform, is well known to be redox activated by thioredoxin f through the reduction of a disulphide bridge involving Cys153 and Cys173. Moreover, data obtained during recent years point to S-nitrosylation as another redox post-translational modification putatively regulating an increasing number of plant enzymes, including cFBP1. In this study we have shown that the Pisum sativum cFBP1 can be efficiently S-nitrosylated by GSNO and SNAP, triggering the formation of the regulatory disulphide. Using in vivo experiments with P. sativum we have established that cFBP1 S-nitrosylation only occurs during the light period and we have elucidated by activity assays with Cys-to-Ser mutants that this enzyme may be inactivated through the S-nitrosylation of Cys153. Finally, in the light of the new data, we have proposed an extended redox-regulation model by integrating the S-nitrosylation and the TRX f-mediated regulation of cFBP1.
URIhttp://hdl.handle.net/10261/167382
Identificadoresdoi: 10.1016/j.redox.2017.10.008
issn: 2213-2317
Aparece en las colecciones: (EEZ) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
2018_Serrato_RB.pdf1,11 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.