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Bacterial Surface Glycans: Microarray and QCM Strategies for Glycophenotyping and Exploration of Recognition by Host Receptors

AutorKalograiaki, Ioanna; Campanero-Rhodes, María Asunción ; Proverbio, Davide; Euba, Begoña; Garmendia, Junkal; Aastrup,Teodor; Solís, D.
Fecha de publicación2018
EditorAcademic Press
CitaciónMethods in Enzymology 598: 37- 70 (2018)
ResumenBacterial surfaces are decorated with a diversity of carbohydrate structures that play important roles in the bacteria¿host relationships. They may offer protection against host defense mechanisms, elicit strong antigenic responses, or serve as ligands for host receptors, including lectins of the innate immune system. Binding by these lectins may trigger defense responses or, alternatively, promote attachment, thereby enhancing infection. The outcome will depend on the particular bacterial surface landscape, which may substantially differ among species and strains. In this chapter, we describe two novel methods for exploring interactions directly on the bacterial surface, based on the generation of bacterial microarrays and quartz crystal microbalance (QCM) sensor chips. Bacterial microarrays enable profiling of accessible carbohydrate structures and screening of their recognition by host receptors, also providing information on binding avidity, while the QCM approach allows determination of binding affinity and kinetics. In both cases, the chief element is the use of entire bacterial cells, so that recognition of the bacterial glycan epitopes is explored in their natural environment.
URIhttp://hdl.handle.net/10261/167269
Identificadoresdoi: 10.1016/bs.mie.2017.06.011
issn: 0076-6879
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