English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/167213
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Redefining nitric oxide production in legume nodules through complementary insights from electron paramagnetic resonance spectroscopy and specific fluorescent probes

AuthorsCalvo-Beguería, Laura ; Rubio Luna, María Carmen ; Martínez, Jesús I.; Pérez-Rontomé, Carmen ; Delgado Igeño, María Jesús; Bedmar, Eulogio J.; Becana Ausejo, Manuel
eElectron paramagnetic resonance
Nitric oxide
Nitrogen fixation
Issue DateJun-2018
PublisherOxford University Press
CitationJournal of Experimental Botany 69(15): 3703-3714 (2018)
AbstractNitric oxide (NO) is a signaling molecule with multiple functions in plants. Given its critical importance and reactivity as a gaseous free radical, we have examined NO production in legume nodules using electron paramagnetic resonance (EPR) spectroscopy and the specific fluorescent dye 4,5-diaminofluorescein diacetate. Also, in this context, we critically assess previous and current views of NO production and detection in nodules. EPR of intact nodules revealed that nitrosyl-leghemoglobin (Lb2+NO) was absent from bean or soybean nodules regardless of nitrate supply, but accumulated in soybean nodules treated with nitrate that were defective in nitrite or nitric oxide reductases or that were exposed to ambient temperature. Consequently, bacteroids are a major source of NO, denitrification enzymes are required for NO homeostasis, and Lb2+NO is not responsible for the inhibition of nitrogen fixation by nitrate. Further, we noted that Lb2+NO is artifactually generated in nodule extracts or in intact nodules not analyzed immediately after detachment. The fluorescent probe detected NO formation in bean and soybean nodule infected cells and in soybean nodule parenchyma. The NO signal was slightly decreased by inhibitors of nitrate reductase but not by those of nitric oxide synthase, which could indicate a minor contribution of plant nitrate reductase and supports the existence of nitrate- and arginine-independent pathways for NO production. Together, our data indicate that EPR and fluorometric methods are complementary to draw reliable conclusions about NO production in plants.
Publisher version (URL)https://doi.org/10.1093/jxb/ery159
Appears in Collections:(EEAD) Artículos
(ICMA) Artículos
(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
redfiprobe.pdf3,85 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.