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QTLs and eQTLs detection for rust resistance in Lathyrus cicera

AutorSantos, C.; Almeida, Nuno Felipe; Alves, Mara Lisa; Rubiales, Diego ; Vaz Patto, María Carlota
Fecha de publicaciónsep-2017
CitaciónInternational Conference Advances in grain legume breeding, cultivations and uses for a more competitive value-chain (2017)
ResumenLathyrus cicera L. (chickling pea) is a cool season legume crop used mainly as animal feed. Chickling pea presents tolerance to abiotic stresses and resistance to some severe diseases, such as rust (Uromyces pisi) and powdery mildew (Erysiphe pisi). However, L. cicera’s resistance mechanisms to these pathogens are still poorly unders tood. As part of the LEGATO European project, we are interested in unveiling the genetic basis and the molecular strategies of resistance against rust in Lathyrus spp.. To accomplish this, genomic and transcriptomic approaches have been integrated on a L. cicera recombinant inbred line (RIL) population, segregating for resistance to this disease. Previously, Quantitative Trait Loci (QTLs) for resistance to rust were mapped in a first version of a L. cicera RILs population linkage map. In the current work, the resolution of this map was improved by adding new EST-SSRs and DArTseq based SNPs. The revised L. cicera genetic linkage map was used to confirm and refine the location of the QTLs previously detected. In addition, the expression levels of five allelic variants (SNPs), within candidate genes for rust resistance previously identified through RNAseq, were quantified by RT-qPCR using in the same RIL population inoculated with U. pisi. These expression data are being used to detect expression QTLs (eQTLs) for rust resistance. The co-localization of QTLs and eQTLs will be discussed. This study will allow a better understanding of the molecular basis of L. cicera’s rust resistance extending considerably the available genomic resources in the Lathyrus genus.
DescripciónTrabajo presentado en la International Conference Advances in grain legume breeding, cultivations and uses for a more competitive value-chain, celebrada en Novi Sad (Serbia) el 27 y 28 de septiembre de 2017.
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