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Age validation in common octopus, Octopus vulgaris using stylet increment analysis

AuthorsHermosilla, Consuelo ; Rocha, Francisco; González, Ángel F. ; Guerra, Ángel ; Fiorito, G.
Issue DateSep-2009
PublisherAcademic Press
CitationCephalopod International Advisory Council Symposium (CIAC’09) ; P25
AbstractDaily periodicity of growth increments in stylets of Octopus vulgaris Cuvier 1797 was validated from known-age laboratory-raised specimens. Daily periodicity of growth increments was corroborated by staining the stylets either with oxytetracycline or tetracycline and comparing the number of rings produced with the elapsed days. A total of 19 individuals (10 males and 9 females, 680 to 1470 g body weight) were marked with oxyetracycline injections at Vigo (mean of 124 mg oxytetracycline per Kg of octopus) and 6 individuals (1 male and 5 females, 248 to 570 g body weight) were stained with tetracycline injections at Naples (mean of 120 mg/Kg). The animals were successfully maintained in captivity until sacrificed for up to 6 (1 specimen), 9 (1 specimen), 18 (6 specimens from Naples) and 21 (17 specimens) days. Transverse stylet sections were embedded in thermo-plastic resin and polished until a thin and translucent section was achieved. Several sections of each stylet were prepared and photographed. Increments were counted 3-5 times by two different readers. Increments counts for each stylet were analysed and outliers excluded using statistical methods. The number of increments counted was 18.9 ± 1.4 and 20.5 ± 1.5 days for 18 and 21 days specimens, respectively. Mean rate of increments formation was 1.02 increments per day (1 increment = 1 day). Photograph analysis corroborated the regular deposition pattern of stylet growth increments. Consequently, this study successfully validates daily increments deposition in stylets of Octopus vulgaris to the size range analysed.
DescriptionContributed to: Cephalopod International Advisory Council Symposium (CIAC’09), Vigo, Spain, Sep 3-11, 2009.-- To be published in the special issue of ICES Journal of Marine Sciences.
Appears in Collections:(IIM) Comunicaciones congresos
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Octopus_vulgaris_Press_release.pdfCSIC Press release (Spanish lang.)44,08 kBAdobe PDFThumbnail
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