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dc.contributor.authorToral, Pablo G.es_ES
dc.contributor.authorHervás, Gonzaloes_ES
dc.contributor.authorLeskinen, H.es_ES
dc.contributor.authorShingfield, Kevin J.es_ES
dc.contributor.authorFrutos, Pilares_ES
dc.date.accessioned2018-06-22T10:27:37Z-
dc.date.available2018-06-22T10:27:37Z-
dc.date.issued2018-
dc.identifier.citationJournal of Dairy Science 101: 6109-6121 (2018)es_ES
dc.identifier.issn1525-3198-
dc.identifier.urihttp://hdl.handle.net/10261/166876-
dc.description13 páginas, 4 figuras, 2 tablas.es_ES
dc.description.abstractA great deal of uncertainty still exists about intermediate metabolites and pathways explaining the biohydrogenation (BH) of 20- and 22-carbon polyunsaturated fatty acids (PUFA). Therefore, this study was conducted to provide further insight into the ruminal metabolism of 20:5 n-3 (EPA), 22:5 n-3 (DPA), and 22:6 n-3 (DHA), the main n-3 PUFA present in the marine lipids used in dairy ruminant feeding, and to examine potential differences between bovine and ovine. To meet this aim, we investigated the 20- and 22-carbon metabolites accumulated during in vitro incubation of EPA, DPA, and DHA with rumen inocula from cows and ewes. The PUFA were added at a dose of 2% incubated dry matter and digesta samples were analyzed after 24 h of incubation using complementary gas-liquid chromatography of fatty acid methyl esters and gas chromatography-mass spectrometry of 4,4-dimethyloxazoline derivatives. Results suggested that the main BH pathway of EPA and DPA would proceed via the reduction of the double bond closest to the carboxyl group (cis-5 in EPA and cis-7 in DPA); curiously, this mechanism seemed of much lower importance for DHA. Thus, DPA would not be a major intermediate product of DHA and their BH might actually follow separate pathways, with the accumulation of numerous unique metabolites in each case. A principal component analysis supported this hypothesis, with a clear separation between PUFA treatments in the score and loading plots. Within EPA and DPA groups, cow and ewe samples loaded separately from each other but not distant. No conjugated 20:5, 22:5, or 22:6 isomer compatible with the initial product of EPA, DPA, or DHA metabolism, respectively, was identified in the ruminal digesta, although this would not unequivocally exclude their transient formation. In this regard, results from DPA incubations provided the first indication that the metabolism of this very long chain PUFA may involve the formation of conjugated double bond structures. The BH of EPA, DPA, and DHA resulted in the appearance of several tentative trans-10-containing metabolites, showing a general trend to be more abundant in the digesta of ewes than in that of cows. This finding was speculated to have some relationship with the susceptibility of dairy sheep to marine lipid-induced milk fat depression. Differences in the relative proportion of intermediate products would also suggest an influence of ruminant species on BH kinetics, with a process that would likely be slower and less complete in cows than in ewes. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO; AGL2014- 54587-R). P. G. Toral benefited from a Ramón y Cajal research contract from the MINECO. Co-funding by the European Regional Development Fund is also acknowledged.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Dairy Science Associationes_ES
dc.relationMINECO/ICTI2013-2016/AGL2014-54587-Res_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectCattlees_ES
dc.subjectMass-spectrometryes_ES
dc.subjectPUFAes_ES
dc.subjectRuminal lipid metabolismes_ES
dc.subjectSheepes_ES
dc.titleIn vitro ruminal biohydrogenation of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic acid (DHA) in cows and ewes: Intermediate metabolites and pathwayses_ES
dc.typeartículoes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3168/jds.2017-14183es_ES
dc.embargo.terms2019-08-01es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.contributor.orcidFrutos, Pilar [0000-0002-4919-5094]es_ES
dc.contributor.orcidToral, Pablo G. [0000-0002-1913-7707]es_ES
dc.contributor.orcidHervás, Gonzalo [0000-0002-0013-7459]es_ES
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