English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/166583
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Structural basis for biologically relevant mechanical stiffening of a virus capsid by cavity-creating or spacefilling mutations

AuthorsGuerra, Pablo ; Valbuena, Alejandro ; Querol-Audí, Jordi ; Silva, Cristina; Castellanos, Milagros; Rodríguez-Huete, Alicia ; Garriga, Damià; Mateu, Mauricio G. ; Verdaguer, Núria
Issue Date22-Jun-2017
PublisherNature Publishing Group
CitationScientific Reports 7: 4101 (2017)
AbstractRecent studies reveal that the mechanical properties of virus particles may have been shaped by evolution to facilitate virus survival. Manipulation of the mechanical behavior of virus capsids is leading to a better understanding of viral infection, and to the development of virus-based nanoparticles with improved mechanical properties for nanotechnological applications. In the minute virus of mice (MVM), deleterious mutations around capsid pores involved in infection-related translocation events invariably increased local mechanical stiffness and interfered with pore-associated dynamics. To provide atomicresolution insights into biologically relevant changes in virus capsid mechanics, we have determined by X-ray crystallography the structural effects of deleterious, mechanically stiffening mutations around the capsid pores. Data show that the cavity-creating N170A mutation at the pore wall does not induce any dramatic structural change around the pores, but instead generates subtle rearrangements that propagate throughout the capsid, resulting in a more compact, less flexible structure. Analysis of the spacefilling L172W mutation revealed the same relationship between increased stiffness and compacted capsid structure. Implications for understanding connections between virus mechanics, structure, dynamics and infectivity, and for engineering modified virus-based nanoparticles, are discussed
Identifiersdoi: 10.1038/s41598-017-04345-w
issn: 2045-2322
Appears in Collections:(IBMB) Artículos
(CBM) Artículos
Files in This Item:
File Description SizeFormat 
663768.pdf6,06 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.