English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/166564
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract

AuthorsGarcía-Villalba, Rocío ; Vissenaekens, Hanne; Pitart, Judit; Romo Vaquero, María ; Espín de Gea, Juan Carlos ; Grootaert, Charlotte; Selma, María Victoria ; Raes, Katleen; Smagghe, Guy; Possemiers, Sam; Van Camp, John; Tomás Barberán, Francisco
KeywordsPhenotypes
Urolithin
Intestinal cells
Gut microbiota
Ellagic acid
Issue Date2017
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 65(27): 5480-5493 (2017)
AbstractA TWIN-SHIME system was used to compare the metabolism of pomegranate polyphenols by the gut microbiota from two individuals with different urolithin metabotypes. Gut microbiota, ellagitannin metabolism, short-chain fatty acids (SCFA), transport of metabolites, and phase II metabolism using Caco-2 cells were explored. The simulation reproduced the in vivo metabolic profiles for each metabotype. The study shows for the first time that microbial composition, metabolism of ellagitannins, and SCFA differ between metabotypes and along the large intestine. The assay also showed that pomegranate phenolics preserved intestinal cell integrity. Pomegranate polyphenols enhanced urolithin and propionate production, as well as Akkermansia and Gordonibacter prevalence with the highest effect in the descending colon. The system provides an insight into the mechanisms of pomegranate polyphenol gut microbiota metabolism and absorption through intestinal cells. The results obtained by the combined SHIME/Caco-2 cell system are consistent with previous human and animal studies and show that although urolithin metabolites are present along the gastrointestinal tract due to enterohepatic circulation, they are predominantly produced in the distal colon region.
Publisher version (URL)https://doi.org/10.1021/acs.jafc.7b02049
URIhttp://hdl.handle.net/10261/166564
DOI10.1021/acs.jafc.7b02049
Identifiersdoi: 10.1021/acs.jafc.7b02049
e-issn: 1520-5118
issn: 0021-8561
Appears in Collections:(CEBAS) Artículos
Files in This Item:
File Description SizeFormat 
gastroinstrack.pdf2,87 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.