English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/166549
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Dynamic cellular maps of molecular species: Application to drug-target interactions

AuthorsGarcía, Carolina ; Losada, A.; Sacristán, M.A.; Martínez-Leal, J.F.; Galmarini, C.M.; Lillo, M.Pilar
Issue Date2018
PublisherNature Publishing Group
CitationScientific Reports 8 (2018)
AbstractThe design of living cell studies aimed at deciphering the mechanism of action of drugs targeting proteins with multiple functions, expressed in a wide range of concentrations and cellular locations, is a real challenge. We recently showed that the antitumor drug plitidepsin (APL) localizes sufficiently close to the elongation factor eEF1A2 so as to suggest the formation of drug-protein complexes in living cells. Here we present an extension of our previous micro-spectroscopy study, that combines Generalized Polarization (GP) images, with the phasor approach and fluorescence lifetime imaging microscopy (FLIM), using a 7-aminocoumarin drug analog (APL) as fluorescence tracer. Using the proposed methodology, we were able to follow in real time the formation and relative distribution of two sets of APL-target complexes in live cells, revealing two distinct patterns of behavior for HeLa-wt and APL resistant HeLa-APL-R cells. The information obtained may complement and facilitate the design of new experiments and the global interpretation of the results obtained with other biochemical and cell biology methods, as well as possibly opening new avenues of study to decipher the mechanism of action of new drugs.
Identifiersdoi: 10.1038/s41598-018-19694-3
issn: 2045-2322
Appears in Collections:(IQFR) Artículos
Files in This Item:
File Description SizeFormat 
s41598-018-19694-3.pdf1,76 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.