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Title

Assessment of Genetic Diversity and Structure of Large Garlic (Allium sativum) Germplasm Bank, by Diversity Arrays Technology “Genotyping-by-Sequencing” Platform (DArTseq)

AuthorsEgea, Leticia A.; Mérida-García, Rosa; Kilian, Andrzej; Hernández Molina, Pilar ; Dorado, Gabriel
KeywordsDNA fingerprinting
Breeding
Phenotype
Somatic mutation
Second-generation sequencing (SGS)
Third-generation sequencing (TGS)
Next-generation sequencing (NGS)
Issue Date20-Jul-2017
PublisherFrontiers Media
CitationFrontiers in Genetics 8: 98 (2017)
AbstractGarlic (Allium sativum) is used worldwide in cooking and industry, including pharmacology/medicine and cosmetics, for its interesting properties. Identifying redundancies in germplasm blanks to generate core collections is a major concern, mostly in large stocks, in order to reduce space and maintenance costs. Yet, similar appearance and phenotypic plasticity of garlic varieties hinder their morphological classification. Molecular studies are challenging, due to the large and expected complex genome of this species, with asexual reproduction. Classical molecular markers, like isozymes, RAPD, SSR, or AFLP, are not convenient to generate germplasm core-collections for this species. The recent emergence of high-throughput genotyping-by-sequencing (GBS) approaches, like DArTseq, allow to overcome such limitations to characterize and protect genetic diversity. Therefore, such technology was used in this work to: (i) assess genetic diversity and structure of a large garlic-germplasm bank (417 accessions); (ii) create a core collection; (iii) relate genotype to agronomical features; and (iv) describe a cost-effective method to manage genetic diversity in garlic-germplasm banks. Hierarchical-cluster analysis, principal-coordinates analysis and STRUCTURE showed general consistency, generating three main garlic-groups, mostly determined by variety and geographical origin. In addition, high-resolution genotyping identified 286 unique and 131 redundant accessions, used to select a reduced size germplasm-bank core collection. This demonstrates that DArTseq is a cost-effective method to analyze species with large and expected complex genomes, like garlic. To the best of our knowledge, this is the first report of high-throughput genotyping of a large garlic germplasm. This is particularly interesting for garlic adaptation and improvement, to fight biotic and abiotic stresses, in the current context of climate change and global warming.
Publisher version (URL)http://dx.doi.org/10.3389/fgene.2017.00098
URIhttp://hdl.handle.net/10261/166543
DOIhttp://dx.doi.org/10.3389/fgene.2017.00098
E-ISSN1664-8021
Appears in Collections:(IAS) Artículos
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