English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/16651
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Characterization of functional bacterial groups in a hypersaline microbial mat community (Salins-de-Giraud, Camargue, France)

AutorFourçans, Aude; García de Oteyza, Tirso; Wieland, Andrea; Solé, Antoni; Diestra, Elia; Van Bleijswijk, Judith; Grimalt, Joan O.; Kühl, Michael; Esteve, Isabel; Muyzer, Gerard; Pierre, Caumette; Duran, Robert
Palabras claveBacterial community composition
Biomarkers
Confocal microscopy
Microbial mat
Microsensors
Bacterial diversity
Fecha de publicación20-ago-2004
EditorFederation of European Microbiological Societies
Blackwell Publishing
CitaciónFEMS Microbiology Ecology 51(1): 55-70 (2004)
ResumenA photosynthetic microbial mat was investigated in a large pond of a Mediterranean saltern (Salins-de-Giraud, Camargue, France) having water salinity from 70‰ to 150‰ (w/v). Analysis of characteristic biomarkers (e.g., major microbial fatty acids, hydrocarbons, alcohols and alkenones) revealed that cyanobacteria were the major component of the pond, in addition to diatoms and other algae. Functional bacterial groups involved in the sulfur cycle could be correlated to these biomarkers, i.e. sulfate-reducing, sulfur-oxidizing and anoxygenic phototrophic bacteria. In the first 0.5 mm of the mat, a high rate of photosynthesis showed the activity of oxygenic phototrophs in the surface layer. Ten different cyanobacterial populations were detected with confocal laser scanning microscopy: six filamentous species, with Microcoleus chthonoplastes and Halomicronema excentricum as dominant (73% of total counts); and four unicellular types affiliated to Microcystis, Chroococcus, Gloeocapsa, and Synechocystis (27% of total counts). Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments confirmed the presence of Microcoleus, Oscillatoria, and Leptolyngbya strains (Halomicronema was not detected here) and revealed additional presence of Phormidium, Pleurocapsa and Calotrix types. Spectral scalar irradiance measurements did not reveal a particular zonation of cyanobacteria, purple or green bacteria in the first millimeter of the mat. Terminal-restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA gene fragments of bacteria depicted the community composition and a fine-scale depth-distribution of at least five different populations of anoxygenic phototrophs and at least three types of sulfate-reducing bacteria along the microgradients of oxygen and light inside the microbial mat.
Descripción16 pages, 8 figures, 2 tables.-- PMID: 16329855 [PubMed].-- Printed version published Dec 27, 2004.
Versión del editorhttp://dx.doi.org/10.1016/j.femsec.2004.07.012
URIhttp://hdl.handle.net/10261/16651
DOI10.1016/j.femsec.2004.07.012
ISSN0168-6496 (Print)
1574-6941 (Online)
Aparece en las colecciones: (IDAEA) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.