Please use this identifier to cite or link to this item:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Characterization of functional bacterial groups in a hypersaline microbial mat community (Salins-de-Giraud, Camargue, France)

AuthorsFourçans, Aude; García de Oteyza, Tirso; Wieland, Andrea; Solé, Antoni; Diestra, Elia; Van Bleijswijk, Judith; Grimalt, Joan O. CSIC ORCID ; Kühl, Michael; Esteve, Isabel; Muyzer, Gerard; Pierre, Caumette; Duran, Robert
KeywordsBacterial community composition
Confocal microscopy
Microbial mat
Bacterial diversity
Issue Date20-Aug-2004
PublisherFederation of European Microbiological Societies
Blackwell Publishing
CitationFEMS Microbiology Ecology 51(1): 55-70 (2004)
AbstractA photosynthetic microbial mat was investigated in a large pond of a Mediterranean saltern (Salins-de-Giraud, Camargue, France) having water salinity from 70‰ to 150‰ (w/v). Analysis of characteristic biomarkers (e.g., major microbial fatty acids, hydrocarbons, alcohols and alkenones) revealed that cyanobacteria were the major component of the pond, in addition to diatoms and other algae. Functional bacterial groups involved in the sulfur cycle could be correlated to these biomarkers, i.e. sulfate-reducing, sulfur-oxidizing and anoxygenic phototrophic bacteria. In the first 0.5 mm of the mat, a high rate of photosynthesis showed the activity of oxygenic phototrophs in the surface layer. Ten different cyanobacterial populations were detected with confocal laser scanning microscopy: six filamentous species, with Microcoleus chthonoplastes and Halomicronema excentricum as dominant (73% of total counts); and four unicellular types affiliated to Microcystis, Chroococcus, Gloeocapsa, and Synechocystis (27% of total counts). Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments confirmed the presence of Microcoleus, Oscillatoria, and Leptolyngbya strains (Halomicronema was not detected here) and revealed additional presence of Phormidium, Pleurocapsa and Calotrix types. Spectral scalar irradiance measurements did not reveal a particular zonation of cyanobacteria, purple or green bacteria in the first millimeter of the mat. Terminal-restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA gene fragments of bacteria depicted the community composition and a fine-scale depth-distribution of at least five different populations of anoxygenic phototrophs and at least three types of sulfate-reducing bacteria along the microgradients of oxygen and light inside the microbial mat.
Description16 pages, 8 figures, 2 tables.-- PMID: 16329855 [PubMed].-- Printed version published Dec 27, 2004.
Publisher version (URL)
Appears in Collections:(IDAEA) Artículos

Show full item record
Review this work


checked on Jun 30, 2022


checked on Jul 3, 2022

Page view(s)

checked on Jul 6, 2022

Google ScholarTM




WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.