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dc.contributor.authorRamirez-Diaz, Diego A.es_ES
dc.contributor.authorGarcía-Soriano, Daniela A.es_ES
dc.contributor.authorRaso, A.es_ES
dc.contributor.authorMücksch, Jonases_ES
dc.contributor.authorFeingold, Marioes_ES
dc.contributor.authorRivas, Germánes_ES
dc.contributor.authorSchwille, Petraes_ES
dc.date.accessioned2018-06-15T11:56:34Z-
dc.date.available2018-06-15T11:56:34Z-
dc.date.issued2018-05-18-
dc.identifier.citationPLoS Biol 16(5):e2004845 (2018)es_ES
dc.identifier.issn1544-9173-
dc.identifier.urihttp://hdl.handle.net/10261/166439-
dc.description20 p.-6 fig.es_ES
dc.description.abstractFtsZ, the primary protein of the bacterial Z ring guiding cell division, has been recently shown to engage in intriguing treadmilling dynamics along the circumference of the division plane. When coreconstituted in vitro with FtsA, one of its natural membrane anchors, on flat supported membranes, these proteins assemble into dynamic chiral vortices compatible with treadmilling of curved polar filaments. Replacing FtsA by a membrane-targeting sequence (mts) to FtsZ, we have discovered conditions for the formation of dynamic rings, showing that the phenomenon is intrinsic to FtsZ. Ring formation is only observed for a narrow range of protein concentrations at the bilayer, which is highly modulated by free Mg2+ and depends upon guanosine triphosphate (GTP) hydrolysis. Interestingly, the direction of rotation can be reversed by switching the mts from the C-terminus to the N-terminus of the protein, implying that the filament attachment must have a perpendicular component to both curvature and polarity. Remarkably, this chirality switch concurs with previously shown inward or outward membrane deformations by the respective FtsZ mutants. Our results lead us to suggest an intrinsic helicity of FtsZ filaments with more than one direction of curvature, supporting earlier hypotheses and experimental evidence.es_ES
dc.description.sponsorshipBMBF/MPG (grant number 031A359A MaxSynBio). The MaxSynBio consortium is jointly funded by the Federal Ministry of Education and Research of Germany and the Max Planck Society. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. German-Israeli Foundation (GIF) (grant number 1160-137.14/2011). to MF and PS. AR is funded through the GIF for Scientific Research and Development. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Graduate School of Quantitative Biosciences of the Ludwig Maximilians University. DR-D and DG-S are supported by a DFG fellowship through QBM. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. JM is supported by the Nanosystems Initiative Munich (NIM) excellence cluster and the International Max Planck Research School for Molecular Life Sciences (IMPRS). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Human Frontiers Science Program (grant number RGP0050/2010). to GR and PS. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Spanish Government (grant number BFU2016-75471-C2-1-P). to GR. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Israel Academy of Science and Humanities (grant number 1701/13). to MF.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.relationMINECO/ICTI2013-2016/BFU2016-75471-C2-1-Pes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.titleTreadmilling analysis reveals new insights into dynamic FtsZ ring architecturees_ES
dc.typeartículoes_ES
dc.identifier.doi10.1371/journal.pbio.2004845-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pbio.2004845es_ES
dc.identifier.e-issn1545-7885-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderFederal Ministry of Education and Research (Germany)es_ES
dc.contributor.funderMax Planck Societyes_ES
dc.contributor.funderGerman-Israeli Foundation for Scientific Research and Developmentes_ES
dc.contributor.funderHuman Frontier Science Programes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderIsrael Academy of Sciences and Humanitieses_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004189es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001736es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003973es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100004412es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100002347es_ES
dc.contributor.orcidMücksch, Jonas [0000-0002-1469-6956]es_ES
dc.contributor.orcidFeingold, Mario [0000-0001-9316-5856 ]es_ES
dc.contributor.orcidRivas, Germán [0000-0003-3450-7478]es_ES
dc.contributor.orcidSchwille, Petra [0000-0002-6106-4847]es_ES
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