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Title

Primer-Independent DNA Synthesis by a Family B DNA Polymerase from Self-Replicating Mobile Genetic Elements

AuthorsRedrejo-Rodríguez, Modesto ; Ordóñez, Carlos D.; Berjón-Otero, Mónica; Aparicio-Maldonado, Cristian; Forterre, patrick; Salas, Margarita ; Krupovic, Mart
Issue Date7-Nov-2017
CitationCell Reports 21: 1574- 1587 (2017)
AbstractFamily B DNA polymerases (PolBs) play a central role during replication of viral and cellular chromosomes. Here, we report the discovery of a third major group of PolBs, which we denote primer-independent PolB (piPolB), that might be a link between the previously known protein-primed and RNA/DNA-primed PolBs. PiPolBs are encoded by highly diverse mobile genetic elements, pipolins, integrated in the genomes of diverse bacteria and also present as circular plasmids in mitochondria. Biochemical characterization showed that piPolB displays efficient DNA polymerization activity that can use undamaged and damaged templates and is endowed with proofreading and strand displacement capacities. Remarkably, the protein is also capable of template-dependent de novo DNA synthesis, i.e., DNApriming activity, thereby breaking the long-standing dogma that replicative DNA polymerases require a pre-existing primer for DNA synthesis. We suggest that piPolBs are involved in self-replication of pipolins and may also contribute to bacterial DNA damage tolerance
URIhttp://hdl.handle.net/10261/166404
Identifiersdoi: 10.1016/j.celrep.2017.10.039
issn: 2211-1247
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