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dc.contributor.authorFernández-García, José L.-
dc.contributor.authorOry, Ana de-
dc.contributor.authorBrussaard, Corina P.D.-
dc.contributor.authorVega, Miguel de-
dc.identifierdoi: 10.1038/s41598-017-07378-3-
dc.identifierissn: 2045-2322-
dc.identifier.citationScientific Reports 7 (2017)-
dc.description.abstractPhaeocystis globosa virus 16T is a giant virus that belongs to the so-called nucleo-cytoplasmic large DNA virus (NCLDV) group. Its linear dsDNA genome contains an almost full complement of genes required to participate in viral base excision repair (BER). Among them is a gene coding for a bimodular protein consisting of an N-terminal Polβ-like core fused to a C-terminal domain (PgVPolX), which shows homology with NAD-dependent DNA ligases. Analysis of the biochemical features of the purified enzyme revealed that PgVPolX is a multifunctional protein that could act as a >Swiss army knife> enzyme during BER since it is endowed with: 1) a template-directed DNA polymerization activity, preferentially acting on DNA structures containing gaps; 2) 5′-deoxyribose-5-phosphate (dRP) and abasic (AP) site lyase activities; and 3) an NAD-dependent DNA ligase activity. We show how the three activities act in concert to efficiently repair BER intermediates, leading us to suggest that PgVPolX may constitute, together with the viral AP-endonuclease, a BER pathway. This is the first time that this type of protein fusion has been demonstrated to be functional.-
dc.description.sponsorshipMinistry of Economy and Competitiveness grant BFU2014-53791-P to M.V., and by an institutional grant from Fundación Ramón Areces to the Centro de Biología Molecular “Severo Ochoa”-
dc.publisherNature Publishing Group-
dc.relation.isversionofPublisher's version-
dc.titlePhaeocystis globosa Virus DNA Polymerase X: A >swiss Army knife>, Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair-
dc.description.versionPeer Reviewed-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderFundación Ramón Areces-
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