English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/166135
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

DC FieldValueLanguage
dc.contributor.authorDíaz-Toledano, Rosa-
dc.contributor.authorLozano, Gloria-
dc.contributor.authorMartínez-Salas, Encarnación-
dc.identifierdoi: 10.1093/nar/gkw795-
dc.identifierissn: 1362-4962-
dc.identifier.citationNucleic Acids Research 45: 1416- 1432 (2017)-
dc.description.abstractThe genome of RNA viruses folds into 3D structures that include long-range RNA–RNA interactions relevant to control critical steps of the viral cycle. In particular, initiation of translation driven by the IRES element of foot-and-mouth disease virus is stimulated by the 3΄UTR. Here we sought to investigate the RNA local flexibility of the IRES element and the 3΄UTR in living cells. The SHAPE reactivity observed in vivo showed statistically significant differences compared to the free RNA, revealing protected or exposed positions within the IRES and the 3΄UTR. Importantly, the IRES local flexibility was modified in the presence of the 3΄UTR, showing significant protections at residues upstream from the functional start codon. Conversely, presence of the IRES element in cis altered the 3΄UTR local flexibility leading to an overall enhanced reactivity. Unlike the reactivity changes observed in the IRES element, the SHAPE differences of the 3΄UTR were large but not statistically significant, suggesting multiple dynamic RNA interactions. These results were supported by covariation analysis, which predicted IRES-3΄UTR conserved helices in agreement with the protections observed by SHAPE probing. Mutational analysis suggested that disruption of one of these interactions could be compensated by alternative base pairings, providing direct evidences for dynamic long-range interactions between these distant elements of the viral genome.-
dc.description.sponsorshipMinisterio de Economia y Competitividad (MINECO) [BFU2014-54564]; Institutional grant from Fundación Ramón Areces-
dc.publisherOxford University Press-
dc.relation.isversionofPublisher's version-
dc.titleIn-cell SHAPE uncovers dynamic interactions between the untranslated regions of the foot-and-mouth disease virus RNA-
dc.description.versionPeer Reviewed-
dc.contributor.funderFundación Ramón Areces-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
Martínez-SalasE_In-CellSHAPEIncovers.pdf12,99 MBAdobe PDFThumbnail
Show simple item record

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.