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Thermosensitive hydrogel platforms with modulated ionic load for optimal cell sheet harvesting

AuthorsMartínez-Campos, Enrique; Santos-Coquillat, A.; Pérez-Ojeda, Maria Eugenia; Civantos, A.; Elvira, Carlos ; Reinecke, Helmut ; García, Carolina ; Ramos, V.; Rodríguez-Hernández, Juan ; Gallardo, Alberto
Cell detachment
Tissue engineering
Issue Date2018
CitationEuropean Polymer Journal 103: 400-409 (2018)
AbstractTissue engineering and regenerative medicine demand affordable and robust technologies for cell manipulation that are capable of culturing cells and collecting them by gentle cell detachment. Nowadays, technologies able to detach and transplant cell monolayers in a controlled and complete manner are particularly relevant. In this work, the capability of vinyl-caprolactam-based hydrogels functionalized with different ionic groups (anionic, cationic or two types of zwitterions) to support mouse endothelial C166-GFP cell growth until confluence and to allow for subsequent cell and cell sheet detachment using thermal stimuli is shown. These hydrogel-type supports, which are robust and easy to ‘handle’ and maneuver, are obtained in a simple, one step radical photopolymerization procedure that requires two types of cross-linkers. For comparison reasons, these formulations were studied together with a neutral hydrogel. Nature and type of charge were shown to greatly influence cell growth. Furthermore, only the neutral and zwitterionic hydrogels exhibited excellent detachment efficiency upon decrease of temperature in spite of the capability of most of the hydrogels to form cell monolayers. The ability of cell sheet detachment was related to the extent of thermosensitivity. The use of hydrogels allowed for transplanting the cells without the need of a superstrate and did not show a thin-thickness requirement, which are usual limitations for the use of poly-N-isopropylacrylamide (pNIPAm) supports, such as commercial grafts. The whole family may actually be considered as a candidate to compete with the expensive and technologically complex thermoresponsive cell platforms based on those pNIPAm grafts.
Publisher version (URL)http://dx.doi.org/10.1016/j.eurpolymj.2018.04.021
Identifiersdoi: 10.1016/j.eurpolymj.2018.04.021
issn: 0014-3057
e-issn: 1873-1945
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