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Title

Structure of promoter-bound TFIID and model of human pre-initiation complex assembly

AuthorsLouder, R.K.; He, Y.; López-Blanco, José R.; Fang, J.; Chacón, Pablo ; Nogales, Eva
Issue Date2016
CitationNature 531: 604- 609 (2016)
AbstractThe general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the core promoter. TFIID comprises the TATA-binding protein (TBP) and 13 TBP-associated factors (TAF1-13), which specifically interact with a variety of core promoter DNA sequences. Here we present the structure of human TFIID in complex with TFIIA and core promoter DNA, determined by single-particle cryo-electron microscopy at sub-nanometre resolution. All core promoter elements are contacted by subunits of TFIID, with TAF1 and TAF2 mediating major interactions with the downstream promoter. TFIIA bridges the TBP-TATA complex with lobe B of TFIID. We also present the cryo-electron microscopy reconstruction of a fully assembled human TAF-less PIC. Superposition of common elements between the two structures provides novel insights into the general role of TFIID in promoter recognition, PIC assembly, and transcription initiation.
URIhttp://hdl.handle.net/10261/166074
Identifiersdoi: 10.1038/nature17394
issn: 1476-4687
Appears in Collections:(IQFR) Artículos
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