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dc.contributor.author | Cano-Linares, María I. | - |
dc.contributor.author | Cabello-Lobato, María J. | - |
dc.contributor.author | González-Prieto, Román | - |
dc.contributor.author | Prado, Félix | - |
dc.date.accessioned | 2018-06-08T09:26:44Z | - |
dc.date.available | 2018-06-08T09:26:44Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | 28th International Conference on Yeast Genetics and Molecular Biology (2017) | - |
dc.identifier.uri | http://hdl.handle.net/10261/165970 | - |
dc.description | Resumen del póster presentado a la 28th International Conference on Yeast Genetics and Molecular Biology (ICYGMB), celebrada en Praga (Czech Republic) del 27 de agosto al 1 de septiembre de 2017. | - |
dc.description.abstract | The recombination proteins Rad51 and Rad52 help the fork to bypass blocking lesions and fill in the gaps of single-stranded DNA (ssDNA) generated during this process of DNA damage tolerance (DDT). In contrast to DNA double-strand breaks, Rad51 and Rad52 recruitment to the ssDNA lesions must occur during S phase. Here we show that Rad51 and Rad52 physically interact with the replicative helicase Mcm2-7 in G1. These interactions are lost during replication unless cells divide in the presence of replicative blocking lesions. They occur mostly in chromatin but are prevented at the pre-RC and at the replication forks, suggesting that Rad51 and Rad52 interact with the excess of Mcm2-7 helicases loaded in G1 and spread to the vicinity of the replication origins. Indeed, Mcm2-7 and Rad51 accumulate at a nuclease-insoluble chromatin fraction enriched in replication factors. Notably, these interactions coordinate the kinetics of chromatin binding of Mcm2-7, Rad51 and Rad52, which accumulate in G1, are released during S/G2 and are maintained in the presence of replicative DNA damage. This chromatin binding behavior is remarkable because homologous recombination is inactive in G1 and active during S/G2. Interestingly, the kinase activity of Cdc7 is required to preserve both the integrity of the Mcm2-7/Rad51/Rad52 complexes and the presence of these factors at chromatin during S/G2. Our results suggest novel roles for Cdc7 and Mcm2-7 in the regulation of the location of recombination proteins during DDT. | - |
dc.rights | closedAccess | - |
dc.subject | Chromatin | - |
dc.subject | Homologous recombination | - |
dc.subject | Replication | - |
dc.title | Physical interactions of Rad51 and Rad52 with Mcm2-7 coordinate their binding to chromatin during the cell cycle and In response to DNA damage | - |
dc.type | póster de congreso | - |
dc.date.updated | 2018-06-08T09:26:45Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.relation.csic | Sí | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6670 | es_ES |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | póster de congreso | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
Aparece en las colecciones: | (CABIMER) Comunicaciones congresos |
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