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Genetic analysis of the role of the Mlp1 export factor in genetic stability

AuthorsGarcía-Benítez, Francisco; Gaillard, Hélène ; Aguilera, Andrés
Issue Date2014
PublisherSociedad Española de Bioquímica y Biología Molecular
CitationXXXVII Congreso SEBBM (2014)
AbstractTranscription of a DNA sequence increases its recombination frequency, a phenomenon referred to as transcription-associated recombination (TAR). During transcription, negative supercoils are accumulated behind the advancing RNA polymerase, facilitating the unwinding of the DNA helix. This transient DNA opening favours the annealing of the nascent mRNA to the transcribed strand (TS) forming a DNA:RNA hybrid (R-loop). R-loops can be formed naturally as intermediates in specific cellular processes, such as mitochondrial DNA replication or immunoglobulin class switching and have been suggested to play a role in transcription termination. A number of observations in E. coli, yeast and humans indicate that the non-transcribed strand (NTS) is more susceptible to damage than the TS. The formation of R-loops, in which the NTS remains single stranded, renders the NTS more vulnerable to DNA damage and contributes to TAR. We are interested in understanding the different mechanisms associated with the optimal biogenesis of mRNA and the control of R-loops formation. We have found mlpD in a screening for deletions of non essential nuclear genes that show hyper recombination when we overexpress a human deaminase that preferentially targets ssDNA in Saccharomyces cerevisiae. The MLP1 gene encodes a nuclear pore basket protein that has an important role in unspliced mRNA retention, SUMO regulation and telomere organization. We investigate the role of Mlp1, its paralog Mlp2 and their human homolog TPR in preventing genomic instability. Current results of Mlp1 will be presented and discussed.
DescriptionResumen del póster presentado al XXXVII Congreso de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Granada del 9 al 12 de septiembre de 2014.
Appears in Collections:(CABIMER) Comunicaciones congresos
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