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Importance of hydrophobic interactions in the single-chained cationic surfactant-DNA complexation

AuthorsLópez-López, Manuel; Checa-Rodriguez, Cintia; Prados-Carvajal, Rosario; Huertas Sánchez, Pablo CSIC ORCID; Moyá, María Luisa
KeywordsCritical micelle concentration
Charge inversion
Single-chained cationic surfactants
Issue Date2018
CitationJournal of Colloid and Interface Science 521: 197-205 (2018)
AbstractThe goal of this work was to understand the key factors determining the DNA compacting capacity of single-chained cationic surfactants. Fluorescence, zeta potential, circular dichroism, gel electrophoresis and AFM measurements were carried out in order to study the condensation of the nucleic acid resulting from the formation of the surfactant-DNA complexes. The apparent equilibrium binding constant of the surfactants to the nucleic acid, K, estimated from the experimental results obtained in the ethidium bromide competitive binding experiments, can be considered directly related to the ability of a given surfactant as a DNA compacting agent. The plot of ln(K) vs. ln(cmc), cmc being the critical micelle concentration, for all the bromide and chloride surfactants studied, was found to be a reasonably good linear correlation. This result shows that hydrophobic interactions mainly control the surfactant DNA compaction efficiency.
DescriptionLópez-López, Manuel et al.
Identifiersdoi: 10.1016/j.jcis.2018.03.048
e-issn: 1095-7103
issn: 0021-9797
Appears in Collections:(CABIMER) Artículos
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