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Bam35 tectivirus intraviral interaction map unveils new function and localization of phage ORFan proteins

AuthorsBerjón-Otero, Mónica; Lechuga, Ana; Mehla, Jetinder; Uetz, Peter; Salas, Margarita CSIC ORCID ; Redrejo-Rodríguez, Modesto CSIC ORCID
KeywordsIntraviral interactome
Yeast two-hybrid
Protein-protein interactions
Issue Date26-Jul-2017
PublisherAmerican Society for Microbiology
CitationJournal of Virology 91 (2017)
AbstractThe family Tectiviridae comprises a group of tailless, icosahedral, membranecontaining bacteriophages that can be divided into two groups by their hosts, either Gram-negative or Gram-positive bacteria. While the first group is composed of PRD1 and nearly identical well-characterized lytic viruses, the second one includes more variable temperate phages, like GIL16 or Bam35, whose hosts are Bacillus cereus and related Gram-positive bacteria. In the genome of Bam35, nearly half of the 32 annotated open reading frames (ORFs) have no homologs in databases (ORFans), being putative proteins of unknown function, which hinders the understanding of their biology. With the aim of increasing knowledge about the viral proteome, we carried out a comprehensive yeast two-hybrid analysis of all the putative proteins encoded by the Bam35 genome. The resulting protein interactome comprised 76 unique interactions among 24 proteins, of which 12 have an unknown function. These results suggest that the P17 protein is the minor capsid protein of Bam35 and P24 is the penton protein, with the latter finding also being supported by iterative threading protein modeling. Moreover, the inner membrane transglycosylase protein P26 could have an additional structural role. We also detected interactions involving nonstructural proteins, such as the DNA-binding protein P1 and the genome terminal protein (P4), which was confirmed by coimmunoprecipitation of recombinant proteins. Altogether, our results provide a functional view of the Bam35 viral proteome, with a focus on the composition and organization of the viral particle.
Identifiersdoi: 10.1128/JVI.00870-17
issn: 1098-5514
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