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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/165541
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dc.contributor.authorLukovic, Dunja-
dc.contributor.authorDíez-Lloret, Andrea-
dc.contributor.authorStojkovic, Petra-
dc.contributor.authorRodríguez Martínez, Daniel-
dc.contributor.authorRodríguez-Jiménez, Francisco Javier-
dc.contributor.authorGonzález-Rodríguez, Patricia-
dc.contributor.authorLópez-Barneo, José-
dc.contributor.authorMoreno-Manzano, Victoria-
dc.contributor.authorStojkovic, Miodrag-
dc.contributor.authorBhattacharya, Shom Shanker-
dc.contributor.authorErceg, Slaven-
dc.date.accessioned2018-06-01T13:07:32Z-
dc.date.available2018-06-01T13:07:32Z-
dc.date.issued2017-
dc.identifierdoi: 10.1002/sctm.16-0371-
dc.identifiere-issn: 2157-6580-
dc.identifierissn: 2157-6564-
dc.identifier.citationStem Cells 6(4): 1217-1226 (2017)-
dc.identifier.urihttp://hdl.handle.net/10261/165541-
dc.descriptionLukovic, Dunja et al.-
dc.description.abstractNeural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid body formation, which decreases efficiency and yield, and strongly limits medical applications. Here we develop a simple, animal-free protocol for neural conversion of both hESCs and hiPSCs in adherent culture conditions. A simple medium formula including insulin induces the direct conversion of >98% of hESCs and hiPSCs into expandable, transplantable, and functional neural progenitors with neural rosette characteristics. Further differentiation of neural progenitors into dopaminergic and spinal motoneurons as well as astrocytes and oligodendrocytes indicates that these neural progenitors retain responsiveness to instructive cues revealing the robust applicability of the protocol in the treatment of different neurodegenerative diseases. The fact that this protocol includes animal-free medium and human extracellular matrix components avoiding embryoid bodies makes this protocol suitable for the use in clinic.-
dc.description.sponsorshipThis work was supported by Wings for Life Foundation, funds for research from the “Miguel Servet” contract of Institute of Health Carlos III of Spanish Ministry of Science and Innovation (CP10/00579) (S.E.), Fund for Health of Spain PI14‐02209 (S.E.), Platform of Biomolecular and Bioinformatics Resources of the Institute of Health Carlos III PT13/0001/0042, Spain (D.L. and S.E.), Czech National Foundation GA CR P304/12/G069 (E.S.), and by the project „BIOCEV “(CZ.1.05/1.1.00/02.0109)” (P.J. and S.E.) and by the European infrastructure for translational medicine (EATRIS‐CZ LM2015064) (E.S.).-
dc.publisherWiley-Blackwell-
dc.publisherAlphaMed Press-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.subjectClinical translation-
dc.subjectCellular therapy-
dc.subjectPluripotent stem cells-
dc.subjectNeural differentiation-
dc.subjectInduced pluripotent stem cells-
dc.subjectEmbryonic stem cells (ESCs)-
dc.subjectDifferentiation-
dc.titleHighly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions-
dc.typeartículo-
dc.identifier.doi10.1002/sctm.16-0371-
dc.relation.publisherversionhttps://doi.org/10.1002/sctm.16-0371-
dc.date.updated2018-06-01T13:07:32Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderCzech Science Foundation-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderEuropean Commission-
dc.relation.csicSí-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004587es_ES
dc.identifier.pmid28213969-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.grantfulltextopen-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.openairetypeartículo-
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