Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/165401
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms |
Autor: | Obolo Mvoulouga, Prosper CSIC ORCID; Oleaga, Ana CSIC ORCID ; Manzano Román, Raúl CSIC ORCID; Pérez Sánchez, Ricardo CSIC ORCID | Palabras clave: | Ornithodoros moubata Mialome Vaccines Midgut antigens Recombinant antigens Synthetic peptides |
Fecha de publicación: | abr-2018 | Editor: | Elsevier | Citación: | Ticks and Tick-borne Diseases (2018) | Resumen: | The African argasid tick Ornithodoros moubata transmits two important pathogens, the African swine fever virus and the spirochete Borrelia duttoni, the cause of human relapsing fever. To date, only conventional control measures such as widespread application of acaricides, strict control measures, and animal movement restrictions have been implemented to confine these diseases. Vaccines against tick infestations have the potential to be among the most efficacious interventions for the management of these diseases. Plasma membrane-associated proteins upregulated in tick midgut cells in response to blood feeding and digestion are thought to play vital functions in tick physiology and in the transmission of tick-borne pathogens. In addition, their antigenic extracellular regions are easily accessible to antibodies synthesised by immunised hosts, which makes them interesting targets for tick vaccine design. The mialomes (midgut transcriptomes and proteomes) of unfed O. moubata females and of engorged females at 48 h post-feeding have recently been obtained, providing a wealth of predicted midgut protein sequences. In the current study, these mialomes were screened using in silico tools to select predicted antigenic transmembrane proteins that were upregulated after feeding (516 proteins). The functionally annotatable proteins from this list (396 proteins) were then manually inspected following additional criteria in order to select a finite and easy-manageable number of candidate antigens for tick vaccine design. The extracellular antigenic regions of five of these candidates were obtained either as truncated recombinant proteins or as KLH-conjugated synthetic peptides, formulated in Freund’s adjuvant, and individually administered to rabbits to assess their immunogenicity and protective potential against infestations by O. moubata and the Iberian species Ornithodoros erraticus. All candidates were highly immunogenic, but provided low protection against the O. moubata infestations (ranging from 7% to 39%). Interestingly, all candidates except one also protected against infestations by O. erraticus, achieving higher efficacies against this species (from 20% to 66%). According to their protective potential, three of the five antigens tested (Om17, Om86 and OM99) were considered little suitable for use in tick vaccines, while the other two (OM85 and OM03) were considered useful antigens for tick vaccine development, deserving further studies. | Descripción: | 64 páginas, 7 figuras, 5 tablas, 4 figuras suplementarias, 3 tablas suplementarias. -- The definitve version is available at: http://www.elsevier.com | Versión del editor: | https://doi.org/10.1016/j.ttbdis.2018.04.015 | URI: | http://hdl.handle.net/10261/165401 | DOI: | 10.1016/j.ttbdis.2018.04.015 | ISSN: | 1877-959X | E-ISSN: | 1877-9603 |
Aparece en las colecciones: | (IRNASA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms.pdf | Artículo principal | 2,42 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
18
checked on 24-mar-2024
WEB OF SCIENCETM
Citations
15
checked on 28-feb-2024
Page view(s)
366
checked on 23-abr-2024
Download(s)
367
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons