English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/165257
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


An ArsR/SmtB family member regulates arsenic resistance genes unusually arranged in Thermus thermophilus HB27

AuthorsAntonucci, Immacolata; Gallo, Giovanni; Limauro, Danila; Contursi, Patrizia; Ribeiro, Ana L.; Blesa, Alba ; Berenguer, José ; Bartolucci, Simonetta; Fiorentino, Gabriella
Issue Date6-Jun-2017
PublisherJohn Wiley & Sons
CitationMicrobial Biotechnology 10: 1690- 1701 (2017)
AbstractArsenic resistance is commonly clustered in ars operons in bacteria; main ars operon components encode an arsenate reductase, a membrane extrusion protein, and an As-sensitive transcription factor. In the As-resistant thermophile Thermus thermophilus HB27, genes encoding homologues of these proteins are interspersed in the chromosome. In this article, we show that two adjacent genes, TtsmtB, encoding an ArsR/SmtB transcriptional repressor and, TTC0354, encoding a Zn/Cd-dependent membrane ATPase are involved in As resistance; differently from characterized ars operons, the two genes are transcribed from dedicated promoters upstream of their respective genes, whose expression is differentially regulated at transcriptional level. Mutants defective in TtsmtB or TTC0354 are more sensitive to As than the wild type, proving their role in arsenic resistance. Recombinant dimeric TtSmtB binds in vitro to both promoters, but its binding capability decreases upon interaction with arsenate and, less efficiently, with arsenite. In vivo and in vitro experiments also demonstrate that the arsenate reductase (TtArsC) is subjected to regulation by TtSmtB. We propose a model for the regulation of As resistance in T. thermophilus in which TtSmtB is the arsenate sensor responsible for the induction of TtArsC which generates arsenite exported by TTC0354 efflux protein to detoxify cells.
Identifiersdoi: 10.1111/1751-7915.12761
issn: 1751-7915
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
BerenguerJ_AnArsR-SmtBFamilyMember.pdf494,75 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.