English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/165043
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Effects of the CTCF transcriptional factor in the erythroid cell differentiation and regulation of erythroid genes

AutorWiesinger, Alexandra
DirectorDelgado, M. Dolores
Fecha de publicación2017
EditorCSIC-IDICAN-UC - Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC)
ResumenCCCTC-binding factor (CTCF) is a highly conserved zinc finger protein, which was first identified as a transcription factor regulating the c-MYC gene. Further studies revealed several new functions of CTCF including regulation of transcription, epigenetics as well as genome architecture. Due to its multivalent functions, it was reported by our group that CTCF also plays a pivotal role during differentiation of human myeloid leukemia cells. In this study, the effects of CTCF on human erythroid differentiation were investigated and CTCF target genes involved in erythroid differentiation were identified and analyzed. As a model system, K562, a pluripotent human leukemia cell line, was utilized. These cells can be induced chemically to differentiation by cytosine arabinoside (Ara-C) and Imatinib. Proliferation, differentiation and apoptosis analysis were performed upon induction. In order to investigate the role of CTCF in differentiation, CTCF was silenced using shRNA. K562 cells were infected with lentiviral particles containing shCTCF and the effect on erythroid differentiation was evaluated. Expression of erythroid markers was analyzed by western blot and RT-qPCR. Furthermore, the binding of CTCF to the regulatory regions of its putative target genes and changes upon differentiation were studied using ChIP (Chromatin Immunoprecipitation). It could be shown that CTCF knock-down inhibited erythroid differentiation of K562 cells. We aimed to extend these studies to primary hematopoietic precursors CD34+ cells purified from cord blood. Erythroid differentiation was induced with erythropoietin. Our data indicate a role of CTCF in the erythroid differentiation of hematopoietic cells.
DescripciónTrabajo fin de Máster
URIhttp://hdl.handle.net/10261/165043
Aparece en las colecciones: (IBBTEC) Tesis
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
CTCF-TFM-Wei.pdf Embargado hasta 10 de julio de 20222,72 MBUnknownVisualizar/Abrir     Petición de una copia
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.