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Studying protein-protein interactions in budding yeast using co-immunoprecipitation

AutorFoltman, Magdalena ; Sánchez-Díaz, Alberto
Palabras claveProtein complexes
Antibody
Freezer/Mill grinder
Cell extract
Protein interactions
Co-immunoprecipitation
Immunoaffinity purification
Magnetic beads
Fecha de publicación2016
EditorSpringer Nature
CitaciónYeast Cytokinesis: 239-256 (2016)
SerieMethods in Molecular Biology 1369
ResumenUnderstanding protein–protein interactions and the architecture of protein complexes in which they work is essential to identify their biological role. Protein co-immunoprecipitation (co-IP) is an invaluable technique used in biochemistry allowing the identification of protein interactors. Here, we describe in detail an immunoaffinity purification protocol as a one-step or two-step immunoprecipitation from budding yeast Saccharomyces cerevisiae cells to subsequently detect interactions between proteins involved in the same biological process.
URIhttp://hdl.handle.net/10261/164957
Identificadoresdoi: 10.1007/978-1-4939-3145-3_17
isbn: 978-1-4939-3144-6
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