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Title

Autoradiographic visualization of G protein-coupled receptors in brain

AuthorsVidal, Rebeca ; Linge, Raquel ; Castillo, María Josefa; Amigó, Josep ; Castro, Elena
Keywords[35S]GTPγS receptor binding in sections
G protein-coupled receptor
Receptor autoradiography
Issue Date2016
PublisherSpringer Nature
CitationReceptor and Ion Channel Detection in the Brain: 143-156 (2016)
SeriesNeuromethods 110
AbstractIn this chapter, two in vitro techniques applied to study the anatomical localization of a receptor are revised: firstly, the receptor binding assay in sections, a technique to assess the location and distribution of a receptor in the tissue (descriptive and anatomical information), and, secondly, the [35S]GTPγS-binding assay also in tissue slices, which allows the quantification of the level of G protein activation by a specific GPCR’s agonist. The latter technique provides additional information about the coupling efficacy of a receptor to G proteins (functional autoradiography), thus enabling the study of the first step in the intracellular signaling pathway after the receptor activation. Both techniques are basically performed for the in vitro autoradiographic visualization of receptors in tissue slices obtained from animal or human postmortem samples.
URIhttp://hdl.handle.net/10261/164944
Identifiersdoi: 10.1007/978-1-4939-3064-7_11
isbn: 978-1-4939-3063-0
Appears in Collections:(IBBTEC) Libros y partes de libros
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