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Título: | Gene expression pattern in above-ground olive tissues upon root colonization by a beneficial bacterium and a pathogenic fungus using a split-root system |
Autor: | Gómez-Lama Cabanás, Carmen CSIC; Sesmero, Rafael CSIC ORCID; Valverde-Corredor, Antonio CSIC ORCID; López-Escudero, Francisco Javier CSIC; Mercado-Blanco, Jesús CSIC ORCID | Palabras clave: | Verticillium dahliae Pseudomonas fluorescens PICF7 Systemic responses |
Fecha de publicación: | abr-2016 | Citación: | 4th Action Meeting: Soil Biodiversity and Ecosystem Services. COST Action FP1305 BioLink (2016) | Resumen: | Pseudomonas fluorescens PICF7 is an indigenous inhabitant of olive roots, displays endophytic lifestyle and exerts effective biocontrol against Verticillium wilt of olive caused by the soil-borne fungus Verticillium dahliae. Previous studies have demonstrated that colonization of olive roots either by strain PICF7 or by V. dahliae triggers differential above-ground transcriptomic changes, mostly related to defense responses to (a)biotic stresses. Some of these responses are common in both interactions. An olive split-root system was developed in order to examine within a 14-day interval the expression pattern of selected genes in above-ground tissues when both microorganisms were spatially separated (i.e. inoculated in different compartments of the split-root system) and sequentially applied (first PICF7, and one week later the pathogen). The final aim is to assess whether PICF7 is able to set up a systemic effective response in the host prior to be challenged by the pathogen. Defense-related genes identified in PICF7-and/or V. dahliae-olive root interactions (i.e. CO-MT, PAL, ACO, CAT, WRKY, 14-3-3 and BRU1) were selected to evaluate by RT-qPCR their expression patterns in aerial tissues when: PICF7 was alone in one root compartment (days 1-14), the pathogen was added to the other compartment (days 7-14), and the pathogen was alone (days 7-14). Differential responses were found depending on the tested gene. Thus, the expression pattern of some genes was not affected regardless the microbial input (e.g. a 14-3-3 protein coding gene). Some showed opposite expression patterns related to the time PICF7 or V. dahliae were inoculated (e.g. a WRKY transcriptional factor). Finally, others only responded upon pathogen’s presence (e.g. BRU1, a brassinosteroid -regulated protein and CO-MT, involved in phenylpropanoid biosynthesis). Ongoing experiments are evaluating whether these expression patterns are similar when the PICF7 and the pathogen are not spatially separated. | Descripción: | Trabajo presentado en el 4th Action Meeting: Soil Biodiversity and Ecosystem Services (COST Action FP1305 BioLink: Linking belowground biodiversity and ecosystem function in European forests), celebrado en Sofía del 12 al 14 de abril de 2016. | URI: | http://hdl.handle.net/10261/164926 |
Aparece en las colecciones: | (IAS) Comunicaciones congresos |
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