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Gene expression pattern in above-ground olive tissues upon root colonization by a beneficial bacterium and a pathogenic fungus using a split-root system

AutorGómez-Lama Cabanás, Carmen ; Sesmero, Rafael; Valverde-Corredor, Antonio ; López-Escudero, Francisco Javier; Mercado-Blanco, Jesús
Palabras claveVerticillium dahliae
Pseudomonas fluorescens
Systemic responses
Fecha de publicaciónabr-2016
Citación4th Action Meeting: Soil Biodiversity and Ecosystem Services. COST Action FP1305 BioLink (2016)
ResumenPseudomonas fluorescens PICF7 is an indigenous inhabitant of olive roots, displays endophytic lifestyle and exerts effective biocontrol against Verticillium wilt of olive caused by the soil-borne fungus Verticillium dahliae. Previous studies have demonstrated that colonization of olive roots either by strain PICF7 or by V. dahliae triggers differential above-ground transcriptomic changes, mostly related to defense responses to (a)biotic stresses. Some of these responses are common in both interactions. An olive split-root system was developed in order to examine within a 14-day interval the expression pattern of selected genes in above-ground tissues when both microorganisms were spatially separated (i.e. inoculated in different compartments of the split-root system) and sequentially applied (first PICF7, and one week later the pathogen). The final aim is to assess whether PICF7 is able to set up a systemic effective response in the host prior to be challenged by the pathogen. Defense-related genes identified in PICF7-and/or V. dahliae-olive root interactions (i.e. CO-MT, PAL, ACO, CAT, WRKY, 14-3-3 and BRU1) were selected to evaluate by RT-qPCR their expression patterns in aerial tissues when: PICF7 was alone in one root compartment (days 1-14), the pathogen was added to the other compartment (days 7-14), and the pathogen was alone (days 7-14). Differential responses were found depending on the tested gene. Thus, the expression pattern of some genes was not affected regardless the microbial input (e.g. a 14-3-3 protein coding gene). Some showed opposite expression patterns related to the time PICF7 or V. dahliae were inoculated (e.g. a WRKY transcriptional factor). Finally, others only responded upon pathogen’s presence (e.g. BRU1, a brassinosteroid -regulated protein and CO-MT, involved in phenylpropanoid biosynthesis). Ongoing experiments are evaluating whether these expression patterns are similar when the PICF7 and the pathogen are not spatially separated.
DescripciónTrabajo presentado en el 4th Action Meeting: Soil Biodiversity and Ecosystem Services (COST Action FP1305 BioLink: Linking belowground biodiversity and ecosystem function in European forests), celebrado en Sofía del 12 al 14 de abril de 2016.
Aparece en las colecciones: (IAS) Comunicaciones congresos
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