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Cell polarity protein Spa2 coordinates chitin synthase II incorporation at the division site in budding yeast

AutorFoltman, Magdalena ; Filali-Mouncef, Yasmina; Crespo, Damaso; Sánchez-Díaz, Alberto
Fecha de publicación2017
CitaciónXL SEBBM Congress (2017)
ResumenDuring cytokinesis cells coordinate actomyosin ring contraction, plasma membrane ingression, secretory vesicle incorporation and remodelling of the extracellular matrix (ECM). In eukaryotes, glycosyltransferases that synthesise ECM polysaccharides are emerging as important players during cytokinesis. In budding yeast the chitin synthase Chs2 makes the primary septum, a special layer of ECM that is essential for cell division. Chs2 is transported on secretory vesicles and its addition to the actomyosin ring must be coordinated with other cytokinetic steps. The process by which the secretory pathway promotes specific incorporation of key factors into the cytokinetic machinery is poorly understood. To try to understand how yeast cells coordinate late cytokinesis steps, we have purified budding yeast Chs2 and the key cytokinesis protein Inn1. We isolated the so called 'ingression progression complexes' or IPCs that contain key actomyosin rings components. We now focus on the cell polarity protein Spa2 that binds to IPCs during cytokinesis. Spa2 interacts with cytokinetic factors Cyk3 and Hof1. Interestingly, Spa2 function turns essential in cells that lack either Cyk3 or Hof1. Those lethal effects can be suppressed by expression of a hypermorphic allele of Chs2, which show the functional relationship between Spa2 and Chs2. Furthermore, Spa2 binds directly to the chitin synthase Chs2. Our findings indicate that Spa2 is recruited to the cleavage site to co-operate with the secretory pathway and actomyosin ring components to promote the incorporation of Chs2 into the IPCs during cytokinesis in budding yeast. We provide new insights into the molecular mechanism by which cells coordinate the secretory pathway, essential for cytokinesis, with the other late cytokinetic steps.
DescripciónResumen del trabajo presentado al XL Congreso de la Sociedad Española de Bioquímica y Biología Molecular (SEBBM), celebrado en Barcelona del 23 al 26 de octubre de 2017.
URIhttp://hdl.handle.net/10261/164862
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