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Comparison of the dissemination of 16S RRNA methyltransferase genes in E. coli from human and natural effluents using whole genome sequencing

AutorDelgado Blas, J. F.; Garcillán-Barcia, M. Pilar; Cruz, Fernando de la ; Gonzalez-Zorn, B.
Fecha de publicación2017
CitaciónFEMS 2017
Resumen[Backgrounds]: The environment plays a critical role in the dissemination of antibiotic resistance genes. The comprehension of the human impact on the acquisition and evolution of these genes is essential to mitigate the burden of antimicrobial resistance (AMR). Whole genome sequencing (WGS) has demonstrated to be highly useful to reveal the epidemiological elements governing the spread of AMR. [Objectives]: The aim of this work was to determine the human influence on the ecology of 16S rRNA methyltransferase genes, which confer high resistance to amynoglicosides, by comparing E. coli from natural and human effluents using WGS. [Methods]: A total of 43 E. coli isolates resistant to amynoglicosides (25 from two waste water treatment plants and 18 from two Spanish rivers near Barcelona) were fully sequenced by Illumina technology (MiSeq). The consequent analysis was performed by the Center for Genomic Epidemiology services. The structure of the methyltransferase gene-carrying plasmids was obtained using PLACNET. [Conclusions]: We have identified two E. coli sequence types (ST479 and ST632) highly disseminated in waste water, which harbored the methyltransferase gene rmtB. However, the isolates recovered from river samples belonged to diverse STs, and all of them carried the methyltransferase gene armA. Furthermore, sewage isolates only shared an IncFII plasmid, while river isolates had a common IncHI2 plasmid. PLACNET analysis critically contributed to understand the resistance gene dynamics in both natural and human environments. These results show the impact of the human activity, which forces the selection of specific genes and plasmids associated to certain STs.
DescripciónResumen del trabajo presentado al 7th Congress of European Microbiologists, celebrado en Valencia (España) del 9 al 13 de julio de 2017.-- Delgado Blas, J. F. et al.
URIhttp://hdl.handle.net/10261/164853
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