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Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike

AuthorsCasas Cendoya, Ana María ; Contreras-Moreira, Bruno ; Pérez Cantalapiedra, Carlos ; Sakuma, Shun; Gracia Gimeno, María Pilar ; Moralejo, María Ángeles; Molina-Cano, José Luis; Komatsuda, Takao; Igartua Arregui, Ernesto
Issue DateMay-2018
CitationCasas AM, Contreras-Moreira B, Cantalapiedra CP, Sakuma S, Gracia MP, Moralejo MA, Molina-Cano JL, Komatsuda T, Igartua E. Resequencing the Vrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike. Molecular Breeding 38: 51 (2018)
AbstractSix-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequencing Vrs1 revealed three two-rowed alleles (Vrs1.b2; Vrs1.b3; Vrs1.t1) and four six-rowed (vrs1.a1; vrs1.a2; vrs1.a3; vrs1.a4) in the natural population. However, the current knowledge about Vrs1 alleles and its distribution among Spanish barley subpopulations is still underexploited. We analyzed the gene in a panel of 215 genotypes, made of Spanish landraces and European cultivars. Among 143 six-rowed accessions, 57 had the vrs1.a1 allele, 83 were vrs1.a2, and three showed the vrs1.a3 allele. Vrs1.b3 was found in most two-rowed accessions, and a new allele was observed in 7 out of 50 two-rowed Spanish landraces. This allele, named Vrs1.b5, contains a ‘T’ insertion in exon 2, originally proposed as the causal mutation giving rise to the six-row vrs1.a2 allele, but has an additional upstream deletion that results in the change of 15 amino acids and a potentially functional protein. We conclude that eight Vrs1 alleles (Vrs1.b2, Vrs1.b3, Vrs1.b5, Vrs1.t1, vrs1.a1, vrs1.a2, vrs1.a3, vrs1.a4) discriminate two and six-rowed barleys. The markers described will be useful for DUS identification, plant breeders, and other crop scientists.
Description24 Pags.- 2 Tabls.- 3 Figs. The definitive version is available at: https://link.springer.com/journal/11032
Publisher version (URL)https://doi.org/10.1007/s11032-018-0816-z
Appears in Collections:(EEAD) Artículos
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