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From buds to paws, a temporal transcriptome analysis

AutorFernández-Guerrero, Marc; Pérez-Gómez, Rocío; Darbellay, Fabrice; Delisle, Lucille; Duboule, Denis; Ros, María A.
Fecha de publicación2016
CitaciónXXXIX Congreso de la SEBBM (2016)
ResumenLimbs develop from limb buds composed of a core of limb progenitor cells covered by the surface ectoderm. A variety of experiments in chick and mouse embryos indicate that the fate of the distal limb progenitors is progressively restricted: early progenitors can form the whole proximo-distal elements of the limb while late progenitors can only form the digits. Here we have used RNA-seq to reveal the transcriptional features associated with this developmental transition. The analysis was performed in mouse forelimbs of 4 different stages (E9.5, E10.5, E11.5 and E12.5) and restricted to the distal 150μm with ectodermal and mesodermal components analyzed separately. Two biological replicates were used for each condition. Of 46,480 Ensembl-annotated genes, 3,416 were differentially expressed between ectoderm and mesoderm. In each tissue, the time serie analysis revealed that major changes occur between E9.5 and E10.5. The accuracy of the expression patterns was confirmed by in situ hybridization and by the truthful coincidence with published expression patterns. According to their temporal expression, six different dynamics of gene expression were identified. In general, the average expression of transcription factors decreased with age, whereas the average expression of extracellular molecules increased with age. The differentially expressed genes were further subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and showed diverse functional categories and signaling pathways. We are exploring their relevance in orchestrating age-specific developmental capacities of each tissue.
DescripciónResumen del póster presentado al XXXIX Congreso de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Salamanca del 5 al 8 de septiembre de 2016.
URIhttp://hdl.handle.net/10261/164759
Aparece en las colecciones: (IBBTEC) Comunicaciones congresos
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