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Title

A New Reversed Phase-HPLC Method Resolving All Major Higher Plant Photosynthetic Pigments

AuthorsDe Las Rivas, Javier ; Abadía Bayona, Anunciación ; Abadía Bayona, Javier
Issue DateSep-1989
PublisherAmerican Society of Plant Biologists
CitationPlant Physiology 91(1): 190-192 (1989)
AbstractA new reversed phase-high performance liquid chromatography method has been developed to analyze the full complement of higher plant photosynthetic pigments (cis-neoxanthin, neoxanthin, violaxanthin, taraxanthin, anteraxanthin, lutein, zeaxanthin, cis-lutein, chlorophyll b, chlorophyll a, - and -carotene). The separation is carried out on a C18 column in about 10 minutes, using a single high-pressure pump and three different mobile phases in three isocratic steps. This method introduces a major improvement in higher plant photosynthetic pigment analysis, resolving in only 10 minutes all photosynthetic pigments while achieving good separation of lutein from its isomer zeaxanthin. Zeaxanthin is involved in the xanthophyll cycle, which recently has been proposed to play a significant role in the protection of the photosynthetic apparatus from photoinhibitory conditions (Demmig et al. [1987] Plant Physiol 84: 218-224).
Publisher version (URL)http://www.plantphysiol.org/cgi/reprint/91/1/190
URIhttp://hdl.handle.net/10261/16466
DOI10.1104/pp.91.1.190
ISSN0032-0889
1532-2548
Appears in Collections:(EEAD) Artículos
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