English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/164648

Relaxases and plasmid transfer in Gram-negative bacteria

AutorZechner, Ellen L.; Moncalián, Gabriel ; Cruz, Fernando de la
Palabras claveRelaxase
DNA helicase
Bacterial conjugation
Bacterial type IV secretion
Conjugative DNA processing
Fecha de publicación2017
EditorSpringer Nature
CitaciónType IV Secretion in Gram-Negative and Gram-Positive Bacteria: 93-113 (2017)
SerieCurrent Topics in Microbiology and Immunology 413
ResumenAll plasmids that spread by conjugative transfer encode a relaxase. That includes plasmids that encode the type IV secretion machinery necessary to mediate cell to cell transfer, as well as mobilizable plasmids that exploit the existence of other plasmids' type IV secretion machinery to enable their own lateral spread. Relaxases perform key functions in plasmid transfer by first binding to their cognate plasmid as part of a multiprotein complex called the relaxosome, which is then specifically recognized by a receptor protein at the opening of the secretion channel. Relaxases catalyze a site- and DNA-strand-specific cleavage reaction on the plasmid then pilot the single strand of plasmid DNA through the membrane-spanning type IV secretion channel as a nucleoprotein complex. In the recipient cell, relaxases help terminate the transfer process efficiently and stabilize the incoming plasmid DNA. Here, we review the well-studied MOBF family of relaxases to describe the biochemistry of these versatile enzymes and integrate current knowledge into a mechanistic model of plasmid transfer in Gram-negative bacteria.
Identificadoresdoi: 10.1007/978-3-319-75241-9_4
isbn: 978-3-319-75240-2
Aparece en las colecciones: (IBBTEC) Libros y partes de libros
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.