English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/164646
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Development of a 16S rRNA-targeted fluorescence in situhybridization probe for quantification of the ammonia-oxidizer Nitrosotalea devanaterra and its relatives

AutorRestrepo-Ortiz, Claudia X.; Merbt, S. ; Barrero-Canossa, J.; Fuchs, B. M.; Casamayor, Emilio O.
Palabras claveCARD-FISHa
Thaumarchaeota
SAGMCG-1 Group
Nitrosotalea devanaterra
Ammonia oxidizing
Soil
Fecha de publicación2018
EditorElsevier
CitaciónSystematic and Applied Microbiology : doi:10.1016/j.syapm.2018.04.003 (2018)
ResumenThe Thaumarchaeota SAGMCG-1 group and, in particular, members of the genus Nitrosotalea have highoccurrence in acidic soils, the rhizosphere, groundwater and oligotrophic lakes, and play a potential rolein nitrogen cycling. In this study, the specific oligonucleotide fluorescence in situ hybridization probeSAG357 was designed for this Thaumarchaeota group based on the available 16S rRNA gene sequencesin databases, and included the ammonia-oxidizing species Nitrosotalea devanaterra. Cell permeabiliza-tion for catalyzed reporter deposition fluorescence in situ detection and the hybridization conditionswere optimized on enrichment cultures of the target species N. devanaterra, as well as the non-targetammonia-oxidizing archaeon Nitrosopumilus maritimus. Probe specificity was improved with a competi-tor oligonucleotide, and fluorescence intensity and cell visualization were enhanced by the design andapplication of two adjacent helpers. Probe performance was tested in soil samples along a pH gradient,and counting results matched the expected in situ distributions. Probe SAG357 and the CARD-FISH pro-tocol developed in the present study will help to improve the current understanding of the ecology andphysiology of N. devanaterra and its relatives in natural environments.
DescripciónEste artículo contiene 6 páginas, 4 figuras, 1 tabla
Versión del editorhttps://doi.org/10.1016/j.syapm.2018.04.003
URIhttp://hdl.handle.net/10261/164646
ISSN0723-2020
E-ISSN1618-0984
Aparece en las colecciones: (CEAB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
in press ortega.pdf456,47 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.