English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/164595
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Analysis of Ras/ERK compartmentalization by subcellular fractionation

AuthorsAgudo-Ibáñez, Lorena; Crespo, Piero ; Casar, Berta
KeywordsSubcellular fractionation
Membrane microdomains
MAP kinases
ERK
Spatial regulation of signaling
Ras
Issue Date2017
PublisherSpringer Nature
CitationERK Signaling: 151-162 (2017)
SeriesMethods in Molecular Biology 1487
AbstractA vast number of stimuli use the Ras/Raf/MEK/ERK signaling cascade to transmit signals from their cognate receptors, in order to regulate multiple cellular functions, including key processes such as proliferation, cell cycle progression, differentiation, and survival. The duration, intensity and specificity of the responses are, in part, controlled by the compartmentalization/subcellular localization of the signaling intermediaries. Ras proteins are found in different plasma membrane microdomains and endomembranes. At these localizations, Ras is subject to site-specific regulatory mechanisms, distinctively engaging effector pathways and switching-on diverse genetic programs to generate a multitude of biological responses. The Ras effector pathway leading to ERKs activation is also subject to space-related regulatory processes. About half of ERK1/2 substrates are found in the nucleus and function mainly as transcription factors. The other half resides in the cytosol and other cellular organelles. Such subcellular distribution enhances the complexity of the Ras/ERK cascade and constitutes an essential mechanism to endow variability to its signals, which enables their participation in the regulation of a broad variety of functions. Thus, analyzing the subcellular compartmentalization of the members of the Ras/ERK cascade constitutes an important factor to be taken into account when studying specific biological responses evoked by Ras/ERK signals. Herein, we describe methods for such purpose.
URIhttp://hdl.handle.net/10261/164595
DOIhttp://dx.doi.org/10.1007/978-1-4939-6424-6_11
Identifiersdoi: 10.1007/978-1-4939-6424-6_11
isbn: 978-1-4939-6422-2
Appears in Collections:(IBBTEC) Libros y partes de libros
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.