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Defined spatiotemporal features of RAS-ERK signals dictate cell fate in MCF-7 mammary epithelial cells

AutorHerrero, Ana; Casar, Berta ; Colón-Bolea, Paula; Agudo-Ibáñez, Lorena; Crespo, Piero
Fecha de publicación2016
EditorJohn Wiley & Sons
Physiological Society (Great Britain)
American Physiological Society
CitaciónMolecular Biology of the Cell 27(12): 1958-1968 (2016)
ResumenSignals conveyed through the RAS-ERK pathway are essential for the determination of cell fate. It is well established that signal variability is achieved in the different microenvironments in which signals unfold. It is also known that signal duration is critical for decisions concerning cell commitment. However, it is unclear how RAS-ERK signals integrate time and space in order to elicit a given biological response. To investigate this, we used MCF-7 cells, in which EGF-induced transient ERK activation triggers proliferation, whereas sustained ERK activation in response to heregulin leads to adipocytic differentiation. We found that both proliferative and differentiating signals emanate exclusively from plasma membrane- disordered microdomains. Of interest, the EGF signal can be transformed into a differentiating stimulus by HRAS overexpression, which prolongs ERK activation, but only if HRAS localizes at disordered membrane. On the other hand, HRAS signals emanating from the Golgi complex induce apoptosis and can prevent heregulin-induced differentiation. Our results indicate that within the same cellular context, RAS can exert different, even antagonistic, effects, depending on its sublocalization. Thus cell destiny is defined by the ability of a stimulus to activate RAS at the appropriate sublocalization for an adequate period while avoiding switching on opposing RAS signals.
Versión del editorhttps://doi.org/10.1091/mbc.E15-02-0118
URIhttp://hdl.handle.net/10261/164482
Identificadoresdoi: 10.1091/mbc.E15-02-0118
e-issn: 1939-4586
issn: 1059-1524
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