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Title

Antibody test for Legionella pneumophila detection

AuthorsPárraga-Niño, Noemí; Quero, Sara; Uria, Naroa; Castillo-Fernandez, Óscar; Jimenez-Ezenarro, Josune; Muñoz Pascual, Francisco Javier; Sabrià, Miquel; García-Nuñez, Marian
KeywordsLegionella
Antibodies
Detection
Cross-reactivity
Issue DateFeb-2018
PublisherElsevier
CitationDiagnostic Microbiology and Infectious Disease 90(2): 85-89 (2018)
AbstractLegionella pneumophila is responsible for Legionnaires' disease (LD). Its detection in both environmental and clinical samples is mainly performed by culture plate method which requires up to 10 days to obtain results. Nowadays, there are commercial antibodies against this bacterium, but they have not been tested against all subgroups of L. pneumophila sg 1 or serogroups 1–16 or their cross-reactions with other non-Legionella bacteria. Indeed, many of these antibodies became available when only 8 serogroups of L. pneumophila had been described. We tested 7 antibodies and found that 2 (Mab 8/5 and OBT) specifically detected all the subgroups of L. pneumophila sg 1, one without cross-reactions (Mab8/5). Moreover, the LP3IIG2 antibody detected almost all serogroups tested with lower rates of cross-reactivity, resulting in a specific sensitive antibody for the detection of L. pneumophila. LP3IIG2 presented higher rate of cross-reactivity against respiratory non-Legionella isolates, thereby contraindicating its clinical applicability.
Publisher version (URL)https://doi.org/10.1016/j.diagmicrobio.2017.11.005
URIhttp://hdl.handle.net/10261/164278
DOI10.1016/j.diagmicrobio.2017.11.005
ISSN0732-8893
E-ISSN1879-0070
Appears in Collections:(IMB-CNM) Artículos

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