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dc.contributor.authorUria, Naroaes_ES
dc.contributor.authorMoral-Vico, Javier-
dc.contributor.authorAbramova, Natalia-
dc.contributor.authorBratov, Andrey-
dc.contributor.authorMuñoz Pascual, Francisco Javier-
dc.date.accessioned2018-04-27T08:33:25Z-
dc.date.available2018-04-27T08:33:25Z-
dc.date.issued2016-04-20-
dc.identifier.citationElectrochimica Acta 198: 249–258 (2016)es_ES
dc.identifier.issn0013-4686-
dc.identifier.urihttp://hdl.handle.net/10261/164267-
dc.description.abstractAs a first stage toward the development of an impedimetric biosensor to quantify viable bacterial cells, this work defines the relationships between microbial growth and impedance changes associated with acidification of the culture medium. Escherichia coli bacterium was used as a model of a common pathogenic bacterium found in a foodstuff. Impedance was measured at a fix frequency of 10 kHz using interdigitated microelectrodes. Impedance changes were found to depend on the number of microorganisms in a sample. However, as the kinetics greatly depends on the bacterial concentration, it was not possible to obtain a single calibration curve in a wide concentration range at a fixed incubation time. To resolve this, a novel calibration method was proposed by measuring the sensor response at 270 and 390 min of incubation and taking a mean value. Obtained calibration plots were used to determine E. coli in a spiked milk samples. Performed experiments demonstrated the capacity of the method to detect E.coli concentrations in a range between 102 and 106 cfu mL−1 in milk in only 6 hours with 5–12% error margin.-
dc.description.sponsorshipAuthors acknowledge financial support from Spanish Ministry of Economy and Competitiveness (project CTQ2014-54553-C3-1-R) and European Union (project ENIAC-ED-52 v161210). This work was partially financially supported by Government of Russian Federation, Grant 074-U01.-
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/CTQ2014-54553-C3-1-Res_ES
dc.relation.isversionofPostprintes_ES
dc.rightsclosedAccesses_ES
dc.subjectIDEA electrodes-
dc.subjectImpedance-
dc.subjectE. coli detection-
dc.subjectCalibration method-
dc.titleFast determination of viable bacterial cells in milk samples using impedimetric sensor and a novel calibration methodes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.electacta.2016.03.060-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://doi.org/10.1016/j.electacta.2016.03.060-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderRussian Government-
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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