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Título

Synchronization of the budding yeast Saccharomyces cerevisiae

AutorFoltman, Magdalena ; Molist, Iago; Sánchez-Díaz, Alberto
Palabras claveSynchronization
G2 and M phases
G1 phase
cdc15-2
Nocodazole
Hydroxyurea
Alpha factor
Saccharomyces cerevisiae
Early S phase
Mitosis
Budding yeast
Fecha de publicación2016
EditorSpringer Nature
CitaciónYeast Cytokinesis. Methods and Protocols: 279-291 (2016)
SerieMethods in Molecular Biology 1369
ResumenA number of model organisms have provided the basis for our understanding of the eukaryotic cell cycle. These model organisms are generally much easier to manipulate than mammalian cells and as such provide amenable tools for extensive genetic and biochemical analysis. One of the most common model organisms used to study the cell cycle is the budding yeast Saccharomyces cerevisiae. This model provides the ability to synchronise cells efficiently at different stages of the cell cycle, which in turn opens up the possibility for extensive and detailed study of mechanisms regulating the eukaryotic cell cycle. Here, we describe methods in which budding yeast cells are arrested at a particular phase of the cell cycle and then released from the block, permitting the study of molecular mechanisms that drive the progression through the cell cycle.
URIhttp://hdl.handle.net/10261/164237
Identificadoresdoi: 10.1007/978-1-4939-3145-3_19
isbn: 978-1-4939-3144-6
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