Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/164229
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dc.contributor.authorRandazzo, Walteres_ES
dc.contributor.authorPiqueras, Joaquínes_ES
dc.contributor.authorRodríguez Díaz, Jesúses_ES
dc.contributor.authorAznar, Rosaes_ES
dc.contributor.authorSánchez Moragas, Gloriaes_ES
dc.date.accessioned2018-04-26T10:37:59Z-
dc.date.available2018-04-26T10:37:59Z-
dc.date.issued2017-06-26-
dc.identifier.citationJournal of Applied Microbiology 124 (4): 958-964 (2018)es_ES
dc.identifier.issn1365-2672-
dc.identifier.urihttp://hdl.handle.net/10261/164229-
dc.description.abstractAim: To improve the efficacy of intercalating dyes to distinguishing between infectious and inactivated hepatitis A virus (HAV) in food. Methods and Results: Different intercalating dyes were evaluated for the discrimination between infectious and thermally inactivated HAV suspensions combining with the RT‐qPCR proposed in the ISO 15216. Among them, PMAxx was the best dye in removing the RT‐qPCR signal from inactivated HAV. Applied to lettuce and spinach, PMAxx–Triton pretreatment resulted in complete removal of the RT‐qPCR signal from inactivated HAV. Likewise, this study demonstrates that this pretreatment is suitable for the discrimination of inactivated HAV in shellfish without further sample dilution. In mussels and oysters, the developed viability RT‐qPCR method reduced the signal of inactivated HAV between 1·7 and 2·2 logs at high inoculation level, and signal was completely removed at low inoculation level. Conclusions: This study showed that the use of PMAxx is an important improvement to assess HAV infectivity by RT‐qPCR. It was shown that PMAxx–Triton pretreatment is suitable for the analysis of infectious HAV in complex food samples such as vegetables and shellfish. Significance and Impact of the Study: The PMAxx–Triton pretreatment can be easily incorporated to the ISO norm for infectious virus detection.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) (RYC‐2012‐09950) and the Spanish National Institute for Agriculture and Food Research and Technology (INIA) co‐financed by the European Social Fund (Project RTA2014‐00024‐C03). Financial support has been co‐sponsored by the European Regional Development Fund (FEDER). G.S. and J.R.D. were supported by the ‘Ramón y Cajal’ Young Investigator.es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.relationMINECO/ICTI2013-2016/RTA2014‐00024‐C03es_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectHepatitis A viruses_ES
dc.subjectViability PCRes_ES
dc.subjectFoodes_ES
dc.subjectWateres_ES
dc.titleImproving efficiency of viability‐qPCR for selective detection of infectious HAV in food and water sampleses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1111/jam.13519-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1111/jam.13519es_ES
dc.embargo.terms2018-06-26es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100007652es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
item.grantfulltextopen-
item.cerifentitytypePublications-
item.openairetypeartículo-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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