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Title

Miniaturized metal oxide pH sensors for bacteria detection

AuthorsUria, Naroa; Abramova, Natalia ; Bratov, Andrey; Muñoz Pascual, Francisco Javier; Baldrich, Eva
KeywordsMicroelectrodes
E. coli detection
Bacterial viability
Tantalum pH sensor
Iridium oxide pH sensor
Issue Date15-Jan-2016
PublisherElsevier
CitationTalanta 147: 364-369 (2016)
AbstractIt is well known that the metabolic activity of some microorganisms results in changes of pH of the culture medium, a phenomenon that can be used for detection and quantification of bacteria. However, conventional glass electrodes that are commonly used for pH measurements are bulky, fragile and expensive, which hinders their application in miniaturized systems and encouraged to the search for alternatives. In this work, two types of metal oxide pH sensors have been tested to detect the metabolic activity of the bacterium Escherichia coli (E. coli). These pH sensors were produced on silicon chips with platinum metal contacts, onto which thin layers of IrOx or Ta2O5 were incorporated by two different methods (electrodeposition and e-beam sputtering, respectively). In order to facilitate measurement in small sample volumes, an Ag/AgCl pseudo-reference was also screen-printed in the chip and was assayed in parallel to an external Ag/AgCl reference electrode. As it is shown, the developed sensors generated results indistinguishable from those provided by a conventional glass pH-electrode but could be operated in significantly smaller sample volumes. After optimization of the detection conditions, the metal oxide sensors are successfully applied for detection of increasing concentrations of viable E. coli, with detection of less than 103 cfu mL−1 in undiluted culture medium in just 5 h.
Publisher version (URL)http://doi.org/10.1016/j.talanta.2015.10.011
URIhttp://hdl.handle.net/10261/164227
DOI10.1016/j.talanta.2015.10.011
ISSN0039-9140
E-ISSN1873-3573
Appears in Collections:(IMB-CNM) Artículos
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