English
español
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/164192
Share/Impact:
Statistics |
![]() ![]() ![]() |
|
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |||
|
Title: | PhcrTx2, a New Crab-Paralyzing Peptide Toxin from the Sea Anemone Phymanthus crucifer |
Authors: | Rodríguez, Armando Alexei; Garateix, Anoland; Salceda, Emilio; Peigneur, Steve; Zaharenko, André Junqueira; Pons, Tirso ; Santos, Yúlica; Arreguín, Roberto; Ständker, Ludger; Forssmann, Wolf-Georg; Tytgat, Jan; Vega, Rosario; Soto, Enrique |
Issue Date: | 7-Feb-2018 |
Publisher: | Multidisciplinary Digital Publishing Institute |
Citation: | Toxins 10 (2): 72 (2018) |
Abstract: | Sea anemones produce proteinaceous toxins for predation and defense, including peptide toxins that act on a large variety of ion channels of pharmacological and biomedical interest. <i>Phymanthus crucifer</i> is commonly found in the Caribbean Sea; however, the chemical structure and biological activity of its toxins remain unknown, with the exception of PhcrTx1, an acid-sensing ion channel (ASIC) inhibitor. Therefore, in the present work, we focused on the isolation and characterization of new <i>P. crucifer</i> toxins by chromatographic fractionation, followed by a toxicity screening on crabs, an evaluation of ion channels, and sequence analysis. Five groups of toxic chromatographic fractions were found, and a new paralyzing toxin was purified and named PhcrTx2. The toxin inhibited glutamate-gated currents in snail neurons (maximum inhibition of 35%, IC<sub>50</sub> 4.7 µM), and displayed little or no influence on voltage-sensitive sodium/potassium channels in snail and rat dorsal root ganglion (DRG) neurons, nor on a variety of cloned voltage-gated ion channels. The toxin sequence was fully elucidated by Edman degradation. PhcrTx2 is a new β-defensin-fold peptide that shares a sequence similarity to type 3 potassium channels toxins. However, its low activity on the evaluated ion channels suggests that its molecular target remains unknown. PhcrTx2 is the first known paralyzing toxin in the family Phymanthidae. |
Publisher version (URL): | https://doi.org/10.3390/toxins10020072 |
URI: | http://hdl.handle.net/10261/164192 |
DOI: | 10.3390/toxins10020072 |
Identifiers: | doi: 10.3390/toxins10020072 |
Appears in Collections: | (CNB) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
toxins-10-00072.pdf | 3,32 MB | Adobe PDF | ![]() View/Open |
Show full item record
Review this work
Review this work
Related articles:
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.