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Furc overexpression leads to variations in the composition and efficiency of photosynthetic apparatus in Anabaena PCC7120

AutorSevilla, E.; Cases, Rafael; Peleato, María Luisa; Fillat, María F.
Fecha de publicación2017
CitaciónFEMS 2017
Resumen[Backgrounds]: Ferric uptake regulator (Fur) proteins are transcriptional regulators that play key roles in many prokariots. Anabaena PCC 7120 contains in its genome three paralogs of Fur proteins whose genes are called all1691 (furA), all2473 (furB) and alr0954 (furC). Until now, little is known about the role of FurC in cyanobacteria. [Objectives]: This work was focused on the study of the functions of FurC protein in Anabaena PCC7120. In order to achieve this goal, an overexpression mutant of FurC protein was constructed in Anabaena PCC7120 rendering the EB2770 strain. [Methods]: Scanning electron microscopy (SEM) studies and growth curves were performed comparing the EB2770 and the wild type strains. Photosynthetic pigments were also determined. Whole-cell absorbance spectra and the fluorescence emission spectra at 77K were carried out in order to compare the composition and efficiency of photosynthetic apparatus in both strains. Finally, pull down experiments were performed with purified FurC protein to identify proteins that could interact with this protein. [Conclusions]: SEM studies revealed changes in size, shape and roughness of the EB2770 cells. Pigment content and yield of energy transfer between the phycobilisome and the photosystem I and II was notably altered in the EB2770 mutant. Pull down results indicated that two linker proteins of phycobilisome interacted with FurC protein. These results suggest that FurC has a role regulating the final composition of photosynthetic pigments and therefore influencing the yield of energy transfer between the phycobilisome and the photosystem I and II although the detailed mechanism remains unknown.
DescripciónResumen del trabajo presentado al 7th Congress of European Microbiologists, celebrado en Valencia (España) del 9 al 13 de julio de 2017.-- et al.
URIhttp://hdl.handle.net/10261/164036
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