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dc.contributor.authorCárcel Márquez, J.es_ES
dc.contributor.authorCamacho, Eva Maríaes_ES
dc.contributor.authorFlores, Amandoes_ES
dc.contributor.authorSantero, Eduardoes_ES
dc.identifier.citationFEMS 2017es_ES
dc.descriptionResumen del trabajo presentado al 7th Congress of European Microbiologists, celebrado en Valencia (España) del 9 al 13 de julio de 2017.es_ES
dc.description.abstract[Backgrounds]: Functional metagenomic allows for the identification of novel activities of interest present in uncultured microorganisms. Unfortunately, this strategy is limited, among other factors, by the accessibility of the heterologous enzyme to the substrate. For intracellular activities, the substrate must enter the bacterial cell to allow detection, while for extracellular activities the host must be able to secrete the enzyme to the growth medium. This way the efficiency of the screening could be limited. [Objectives]: The main purpose of this work is to develop an autolysis system that, in response to an inducer, lysates bacteria releasing their content into the growth medium. This system must also permit survival of a proportion of the bacterial population to allow the recovery of positive clones. [Methods]: We have implemented an inducible autolysis system previously developed in our laboratory. It is based in the lysis operon of lambda phage and responds to anhydrotetracycline as an inducer. We have demonstrated that the autolysis system allows detection of intracellular activities and the recovery of positive clones. Subsequently we carried out a screening for cellullase activity in the presence or absence of the autolysis system to compare the efficiency of cellulose activity detection. [Conclusions]: We have demonstrated that the autolysis system is necessary for the detection of intracellular activities. Additionally, the system improves detection of positive clones for cellullase activity. In all cases, the lysis system allows recovery and subsequent confirmation of positive clones. In summary, our system is an effective tool to improve functional metagenomic analysis.es_ES
dc.titleImplementation of an autolysis systems as a tool for functional metagenomics analysises_ES
dc.typecomunicación de congresoes_ES
dc.description.peerreviewedPeer reviewedes_ES
oprm.item.hasRevisionno ko 0 false*
Appears in Collections:(CABD) Comunicaciones congresos
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