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Título

SADB phosphorylation of γ-tubulin regulates centrosome duplication

AutorAlvarado-Kristensson, María; Rodríguez, María Josefa; Silió, Virginia; Valpuesta, José M.; Carrera, Ana C.
Fecha de publicación2-ago-2009
EditorNature Publishing Group
CitaciónNature Cell Biology 11(9): 1081-1092 (2009)
ResumenSymmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a short SADB Ser/Thr kinase whose activity fluctuates during the cell cycle, localizes to centrosomes, and controls centrosome duplication. Reduction of endogenous SADB levels diminished centrosome numbers, whereas enhanced SADB expression induced centrosome amplification. SADB exerted this action through phosphorylation of γ-tubulin on Ser 131, as expression of a phosphomimetic Ser 131-to-Asp γ-tubulin mutant alone increased centrosome numbers, whereas non-phosphorylatable Ala 131-γ-tubulin impaired centrosome duplication. We propose that SADB kinase activity controls centrosome homeostasis by regulating phosphorylation of γ-tubulin.
Descripción17 pages, 11 figures.-- PMID: 19648910 [PubMed].-- Printed version published Sep 2009.
Supporting information available at: http://www.nature.com/ncb/journal/vaop/ncurrent/suppinfo/ncb1921_S1.html
Versión del editorhttp://dx.doi.org/10.1038/ncb1921
URIhttp://hdl.handle.net/10261/16397
DOI10.1038/ncb1921
ISSN1465-7392 (Print)
1476-4679 (Online)
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